Syd Labs D-luciferin potassium salt MB000102-R70170 正确应用提升实验效果

Syd Labs D-luciferin potassium salt如何应用提升实验效果？看这些应用文献就够了！

D-luciferin potassium salt如何提升实验效果？D-luciferin potassium salt（D-荧光素钾盐）在生物学和医学研究中的实验效果，尤其是活体成像实验中，可以通过以下几个方面进行提升：选择高纯度和高质量的D-luciferin potassium salt（比如美国Syd Labs 货号MB000102-R70170或MB102，进口品牌D-luciferin potassium salt价格最低）、正确的储存与制备、合理的实验设计与操作、动力学研究与优化，D-荧光素钾盐文献参考。通过以上，可以显著提升D-luciferin potassium salt在实验中的效果，为生物学和医学研究提供更加准确和可靠的数据支持。

下面我们一起来看看Syd Labs MB000102-R70170 D-luciferin potassium salt应用文献，看看在具体的实验中如何使用了D-荧光素钾盐：

Engineering Circulating Tumor Cells as Novel Cancer Theranostics

Katie M Parkins, Veronica P Dubois, John J Kelly , Yuanxin Chen , Natasha N Knier, Paula J Foster, John A Ronald

Theranostics. 2020 Jun 29;10(17):7925-7937. doi: 10.7150/thno.44259. eCollection 2020.

All in vitro results are from three independent experiments with three replicates of each condition. To evaluate the relationship between cell number and BLI signal, 1×104, 5×104, 1×105, 1.5×105, and 5×105 4T1BR5-FLuc or 4T1-RLuc cells were seeded in each well of 24-well plates. We acquired fluorescent images of each plate. We then added 10 μL of D-luciferin (30 mg/mL; Syd Labs, Inc., MA, USA) or 10 μL of h-Coelenterazine (150μg/mL; NanoLight Technology, Prolume, AZ, USA) to the growth medium in each well and BLI images were collected for up to 35 minutes. All images were acquired using a hybrid optical/X-ray scanner (IVIS Lumina XRMS In Vivo Imaging System, PerkinElmer). Signal was measured with region-of-interest (ROI) analysis using LivingImage Software (Perkin Elmer). An ROI was drawn around each well to measure the radiant efficiency (p/s/cm2/sr/uW/cm2) for fluorescence images and average radiance (p/s/mm2/sr) for bioluminescence images. The mean signal across replicates was determined for each independent experiment.

Cellular MRI reveals altered brain arrest of genetically engineered metastatic breast cancer cells

Katie M Parkins, Amanda M Hamilton , Veronica P Dubois, Suzanne M Wong , Paula J Foster, John A Ronald

Contrast Media Mol Imaging. 2019 Jan 8:2019:6501231. doi: 10.1155/2019/6501231. eCollection 2019.

BLI was performed using a hybrid optical/X-ray scanner (IVIS Lumina XRMS In Vivo Imaging System, PerkinElmer). Mice were anesthetized with isofluorane (2% in 100% oxygen) and received a 150 μL intraperitoneal injection of D-luciferin (30 mg/mL; Syd Labs, Inc., MA, USA), and BLI images were captured for up to 35 minutes after injection.