重组抗Myc-Tag单克隆抗体（克隆号9E10），小鼠 IgG1 Kappa | Syd Labs PA000002.m1

描述

了解更多抗Myc单抗（抗Myc-Tag单抗，抗c-Myc抗体，c-Myc抗体）（clone：9E10）引用文献，请查看：Myc抗体（克隆号9E10）引用文献

Myc-9E10抗体参考文献:

1. Truncated mini LRP1 transports cargo from luminal to basolateral side across the blood brain barrier
Laura Fritzen,et al.Fluids Barriers CNS. 2024.PMCID: PMC11409491
“Background:The most crucial area to focus on when thinking of novel pathways for drug delivery into the CNS is the blood brain barrier (BBB). A number of nanoparticulate formulations have been shown in earlier research to target receptors at the BBB and transport therapeutics into the CNS. However, no mechanism for CNS entrance and movement throughout the CNS parenchyma has been proposed yet. Here, the truncated mini low-density lipoprotein receptor-related protein 1 mLRP1_DIV* was presented as blood to brain transport carrier, exemplified by antibodies and immunoliposomes using a systematic approach to screen the receptor and its ligands’ route across endothelial cells in vitro.Methods:The use of mLRP1_DIV* as liposomal carrier into the CNS was validated based on internalization and transport assays across an in vitro model of the BBB using hcMEC/D3 and bEnd.3 cells. Trafficking routes of mLRP1_DIV* and corresponding cargo across endothelial cells were analyzed using immunofluorescence. Modulation of γ-secretase activity by immunoliposomes loaded with the γ-secretase modulator BB25 was investigated in co-cultures of bEnd.3 mLRP1_DIV* cells and CHO cells overexpressing human amyloid precursor protein (APP) and presenilin 1 (PSEN1).Results:We showed that while expressed in vitro, mLRP1_DIV* transports both, antibodies and functionalized immunoliposomes from luminal to basolateral side across an in vitro model of the BBB, followed by their mLRP1_DIV* dependent release of the cargo. Importantly, functionalized liposomes loaded with the γ-secretase modulator BB25 were demonstrated to effectively reduce toxic Aß42 peptide levels after mLRP1_DIV* mediated transport across a co-cultured endothelial monolayer.Conclusion:Together, the data strongly suggest mLRP1_DIV* as a promising tool for drug delivery into the CNS, as it allows a straight transport of cargo from luminal to abluminal side across an endothelial monolayer and it’s release into brain parenchyma in vitro, where it exhibits its intended therapeutic effect.Supplementary Information:The online version contains supplementary material available at 10.1186/s12987-024-00573-1.”
2. A multi-Fc-species system for recombinant antibody production
Sandrine Moutel,et al.BMC Biotechnol. 2009.PMCID: PMC2654441
“Background：Genomic, transcriptomic and proteomic projects often suffer from a lack of functional validation creating a strong demand for specific and versatile antibodies. Antibody phage display represents an attractive approach to select rapidly in vitro the equivalent of monoclonal antibodies, like single chain Fv antibodies, in an inexpensive and animal free way. However, so far, recombinant antibodies have not managed to impose themselves as efficient alternatives to natural antibodies.Results：We developed a series of vectors that allow one to easily fuse single chain Fv antibodies to Fc domains of immunoglobulins, improving their sensitivity and facilitating their use. This series enables the fusion of single chain Fv antibodies with human, mouse or rabbit Fc so that a given antibody is no longer restricted to a particular species. This opens up unlimited multiplexing possibilities and gives additional value to recombinant antibodies. We also show that this multi-Fc species production system can be applied to natural monoclonal antibodies cloned as single chain Fv antibodies and we converted the widely used 9E10 mouse anti-Myc-tag antibody into a human and a rabbit antibody.Conclusion：Altogether, this new expression system, that brings constant quality, sensitivity and unique versatility, will be important to broaden the use of recombinant and natural monoclonal antibodies both for laboratory and diagnosis use.”
3. Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes
Suzie J. Scales,et al.J Am Soc Nephrol. 2020.PMCID: PMC7461670
“Specific variants of APOL1, G1 and G2, are associated with CKD in the Black population. Overexpression of these variants kills cells, through different proposed mechanisms in different subcellular compartments. The localization of endogenous APOL1 has not been conclusively established because all studies have used antibodies that crossreact with APOL2. Generation and use of APOL1-specific antibodies show that endogenous podocyte APOL1 localizes mainly inside the endoplasmic reticulum, with a few molecules on the cell surface. These findings potentially support the endoplasmic reticulum stress or cell surface cation channel models of cytotoxicity.”

重组抗Myc-Tag单克隆抗体，小鼠 IgG1 Kappa（克隆号9E10，货号：PA000002.m1）

重组抗Myc-Tag单克隆抗体（克隆 9E10），小鼠 IgG1 Kappa是用哺乳动物细胞生产的重组抗体，由小鼠可变区和小鼠 IgG1 κ 不变区组成，适用于体外和体内研究，包括蛋白质纯化、WB、IP和IF等。

其它抗Myc抗体包括:Myc-tag单克隆抗体（Myc-tag Monoclonal Antibody）、c-Myc-tag单克隆抗体（c-Myc-tag Monoclonal Antibody）、偶联磁珠Myc-tag抗体（Magnetic Microbead-Conjugated Myc-tag Monoclonal Antibody）、琼脂糖Myc-tag单抗（Agarose-Conjugated Myc-tag Monoclonal Antibody）。Myc标签抗体信息可以通过duozhaozhao.com获取更多信息，包括抗Myc标签抗体试用装活动信息等。

抗Myc标签抗体相关活动信息和资讯：

新春特惠 | 悉得标签抗体买一送一
寻访Myc标签抗体测评科学家
Myc标签抗体（克隆号9E10）为什么最受欢迎？
Myc标签抗体有何优势？
Myc标签抗体的特异性解析
Myc标签抗体在蛋白结晶中的作用及去除方法
Myc标签抗体是否适用于所有表达系统？
Myc标签抗体与哪些蛋白可以融合表达？
Myc标签抗体在Western blot中的使用技巧
Myc标签抗体如何进行亲和纯化？
Myc标签抗体在科研中有何应用？
Myc标签抗体是什么？

Syd Labs还提供如下常用的标签抗体:

重组抗DYKDDDDK-Tag单克隆抗体(克隆号M2.1)，小鼠IgG1 Kappa
重组抗HA-Tag单克隆抗体(克隆号12CA5)，小鼠IgG2b Kappa
重组抗His-Tag单克隆抗体(克隆号3D5)，小鼠IgG2b Kappa
重组GFP-tag 单克隆抗体
重组RFP-tag 单克隆抗体
重组V5-tag 单克隆抗体

请记住我们的产品信息: 重组抗Myc-Tag单克隆抗体（克隆号9E10），小鼠 IgG1 Kappa: PA000002.m1 Syd Labs Recombinant Anti-Myc-Tag Monoclonal Antibody (Clone 9E10), Mouse IgG1 Kappa。