重组小鼠IgG3同型对照抗体,体内实验级 | 悉得(Syd Labs) PA007161
悉得(Syd Labs)体内实验级重组小鼠IgG3同型对照抗体(货号:PA007161)是用哺乳动物细胞生产的重组抗体,与经测试的小鼠样本的特异性结合程度低或无特异性结合,还有更多重组小鼠IgG3同型对照抗体相关产品,包括各种靶标、突变体、标签和偶联物。重组小鼠IgG3 kappa同型对照抗体适合体外和体内研究。
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货号 | PA007161 |
---|---|
产品名称 | 重组小鼠IgG3同型对照抗体,体内实验级 | 悉得(Syd Labs) PA007161 |
英文名 | In Vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody |
供货商名称 | Syd Labs, Inc. |
品牌名 | 悉得(Syd Labs) |
别称 | 重组小鼠IgG3同型对照,小鼠IgG3阴性对照抗体,mIgG3同型对照抗体 |
概述 | 重组小鼠IgG3 kappa同型对照抗体适合体外和体内研究。与经测试的小鼠样本的特异性结合程度低或无特异性结合。我们还提供更多重组小鼠IgG3同型对照抗体相关产品,包括各种靶标、突变体、标签和偶联物。 |
克隆号 | 12B9 |
同种型 | 小鼠 IgG3, kappa |
应用 | 用于ELISA、蛋白质印迹 (WB)、流式细胞术(Flow)、免疫沉淀(IP)、免疫组织化学(石蜡)(IHC (P))、免疫组织化学(Frozen) (IHC (F))和体内动物模型研究的小鼠IgG3 kappa抗体的同型匹配阴性对照。 |
免疫源 | N/A |
抗体形式 | 0.2 μM过滤溶液,1x PBS |
内毒素 | 根据 LAL 方法,≤ 1 EU每 1 mg 蛋白质。 |
纯度 | >95%(在还原条件下通过SDS-PAGE测定) |
运输 | 体内实验级重组小鼠IgG3同型对照抗体用冰袋运输。收到后,请立即将其存放在下面建议的温度下。 |
稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 如果保存在2 至 8°C,自收到之日起可保存3个月。如果保存在-20 至 -70°C,自收到之日起可保存 12个月。 |
注意事项 | PA007161 悉得(Syd Labs)重组小鼠IgG3 kappa同型对照抗体适合体外和体内研究。与经测试的小鼠样本的特异性结合程度低或无特异性结合。悉得(Syd Labs)还提供更多重组小鼠IgG3同型对照相关产品,包括各种靶标、突变体、标签和偶联物。 |
产品咨询 | 悉得(Syd Labs)在国内只通过代理商销售其产品,不做直销。终端用户咨询价格请联系悉得(Syd Labs)中国代理商。 关于悉得(Syd Labs)产品如果有任何技术或其它问题,欢迎随时联系悉得(Syd Labs)国内市场推广合作伙伴:武汉多找找科技有限公司,企业微信:duozhaozhao2024 联系电话:18162581039(龙经理) |
描述
PA007161: 重组小鼠IgG3同型对照抗体,体内实验级(In Vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody)
背景知识
Mouse IgG3 isotype control(小鼠IgG3同型对照抗体) (natural and engineered mIgG3 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study.
Please contact us to ask for a quote for the Fc silenced mouse IgG3 isotype control mutants(Fc silenced小鼠IgG3同型对照突变体), mIgG3 isotype control and mutants(mIgG3同型对照抗体和突变体) with Avi-, His-, and Flag-tags, and biotinylated mIgG3 isotype control and mutants(mIgG3同型对照抗体和突变体). A variety of conjugates (such as dyes and fluorophores) with the mouse IgG3 isotype control and Fc-slient mutants(小鼠IgG3同型对照抗体和fc slient突变体) are available.
Mouse IgG antibodies(小鼠IgG抗体) are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a or IgG2c, IgG2b, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.
Questions and Answers:
Question: What is the advantage of the recombinant antibody production method compared to the hybridoma method?
Answer: We use chemically defined medium for cell culturing to produce the recombinant rat IgG isotype controls(重组大鼠IgG同型对照抗体). There is significant advantage for the in vitro method. First, we do not use mouse ascites for antibody production. Rat hybridoma cell lines usually adapt poorly to in vitro conditions, and generate ascites in immunocompromised mice rather than rats. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some companies and scientists add FBS into hybridoma cell culturing medium, which results in antibody contamination. There is no such contamination in production of recombinant antibodies. Fourth, we can easily switch the isotype with different antibody constant regions, and generate Fc mutations such as D265A which is used in therapeutic antibody research. It is better to use the same isotype control mutation.
Question: What is an isotype control antibody and why is it used?
Answer: An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to differentiate, if there is any, non-specific background signal from the specific antibody signal. Mouse IgG1 kappa isotype controls against KLH, TNP, DNP, GST, HEL, His-tag, Flag, c-myc, HA, and GFP meet the requirements in some cases but do not in specific cases in the presence of the targets or cross-reactivity.
悉得(Syd Labs)提供以下体内实验级重组小鼠IgG3同型对照抗体(In Vivo Grade Recombinant Mouse IgG3 Isotype Control Antibodies):
重组小鼠IgG3同型对照抗体,体内实验级(In Vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody)
悉得(Syd Labs)其它体内实验级重组IgG同型对照抗体和突变体(In vivo grade Recombinant IgG Isotype Control Antibodies and Mutants):
重组人IgG1同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants)
重组人IgG2同型对照抗体,体内实验级(In vivo Grade Recombinant Human IgG2 Isotype Control Antibody)
重组人IgG3同型对照抗体,体内实验级(In vivo Grade Recombinant Human IgG3 Isotype Control Antibody)
重组人IgG4-S228P同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants)
重组小鼠IgG1同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants)
重组小鼠IgG2a同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants)
重组小鼠IgG2b同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants)
重组小鼠IgG2c同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants)
重组小鼠IgG3同型对照抗体,体内实验级(In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody)
重组大鼠IgG1同型对照抗体,体内实验级(In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody)
重组大鼠IgG2a同型对照抗体,体内实验级(In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody)
重组大鼠IgG2b同型对照抗体,体内实验级(In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody)
重组大鼠IgG2c同型对照抗体,体内实验级(In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody)
重组仓鼠IgG1同型对照抗体,体内实验级(In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody)
重组仓鼠IgG2同型对照抗体,体内实验级(In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody)
悉得(Syd Labs)体内实验级重组IgG Fc蛋白(In vivo Grade Recombinant IgG Fc Proteins):
重组人IgG1 Fc蛋白(hIgG1),体内实验级(In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1))
重组人IgG2 Fc蛋白(hIgG2),体内实验级(In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2))
重组人IgG4 Fc蛋白(hIgG4),体内实验级(In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4))
重组小鼠IgG1 Fc蛋白(mIgG1),体内实验级(In vivo Grade Recombinant Mouse IgG1 Fc Protein(mIgG1))
重组小鼠IgG2a Fc蛋白(mIgG2a),体内实验级(In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a))
重组小鼠IgG2b Fc蛋白(mIgG2b),体内实验级(In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b))
重组大鼠IgG2a Fc蛋白(rtIgG2a),体内实验级(In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a))
重组大鼠IgG2b Fc蛋白(rtIgG2b),体内实验级(In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b))
重组羊驼IgG2b Fc蛋白(lIgG2b),体内实验级(In vivo Grade Recombinant Llama IgG2b Fc Protein(lIgG2b))
重组兔IgG Fc蛋白(rIgG),体内实验级(In vivo Grade Recombinant Rabbit IgG Fc Protein(rIgG))
请记住我们的产品信息: 体内实验级重组小鼠IgG3同型对照抗体: PA007161 Syd Labs In Vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody。