抗小鼠NK1.1单克隆抗体(克隆号PK136) ，体内实验级重组，小鼠IgG2a Kappa | Syd Labs PA007525.m2a
体内实验级重组抗小鼠NK1.1单克隆抗体，小鼠IgG2a Kappa（克隆号：PK136，货号：PA007525.m2a）是用哺乳动物细胞生产的重组抗体，纯度>95%，适用于体外和体内研究，比如体内NK细胞耗竭（清除）。Syd Labs PA007525.m2a不变区为小鼠Mouse IgG2a Kappa (mIgG2a或m2a)，可与重组小鼠IgG2a同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
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| 货号 | PA007525.m2a |
|---|---|
| 产品名称 | 抗小鼠NK1.1单克隆抗体(克隆号PK136) ，体内实验级重组，小鼠IgG2a Kappa | Syd Labs PA007525.m2a |
| 英文名 | In Vivo Grade Recombinant Anti-mouse NK1.1 Monoclonal Antibody (Clone: PK136), Mouse IgG2a Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | KLRB1C, CD161, Ly-59, NK-RP1, NK-1, Nk-1.2, Nkrp1c, Ly-55c |
| 概述 | Syd Labs 重组抗小鼠NK1.1单克隆抗体(克隆：PK136) 是用哺乳动物细胞生产的重组抗体，可与重组小鼠IgG2a同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | PK136 |
| 同种型 | 小鼠 IgG2a Kappa |
| 特异性 | 体内实验级重组小鼠单克隆抗体 (克隆：PK136) 特异性与小鼠 NK1.1 结合 |
| 应用 | ELISA、中和、功能测定，如生物分析PK和ADA测定，以及用于研究受小鼠NK1.1蛋白影响的生物途径的测定 |
| 抗体形式 | 0.2 μM过滤溶液，pH 7.4，不含稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗小鼠NK1.1单克隆抗体（克隆号PK136），小鼠IgG2a Kappa用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007525.m2a Syd Labs：抗小鼠NK1.1单克隆抗体是用哺乳动物细胞生产的，可与重组小鼠IgG2a同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
描述
PA007525.m2a: Syd Labs体内实验级重组抗小鼠NK1.1单克隆抗体（克隆号PK136），小鼠IgG2a Kappa（In Vivo Grade Recombinant Anti-mouse NK1.1 Monoclonal Antibody (Clone: PK136), Mouse IgG2a Kappa）
抗小鼠NK1.1抗体（PK136）部分参考文献:
FTO negatively regulates the cytotoxic activity of natural killer cells
Wang X, et al. EMBO J. 2023 Apr 3;42(7):e111932. doi: 10.15252/embj.2022111932. PMID: 36744362
The anti-NK1.1 antibody (PK136) was used to deplete NK cells in mice via intraperitoneal injection, with IgG control antibodies as a comparator (n=2/group). NK cell depletion was confirmed by flow cytometry, and metastatic burden in the lung was assessed after B16F10 cell injection, with PK136 or IgG control administered on days -4, -1, and 2. Loss of Fto expression was shown to enhance NK cell activity, and PK136 injection efficiently depleted NK cells in vivo, as detailed in Figure EV2.
Tags: anti-mouse NK1.1 PK136; anti-mouse NK1.1 PK136 antibody
Role of NK Cells in Skin Wound Healing of Mice
Chen Y, et al. Int J Mol Sci. 2023 Mar 31;24(7):6573. doi: 10.3390/ijms24076573. PMID: 36883869
Mice subjected to full-thickness wounding were injected intraperitoneally with 100 µg of anti-NK1.1 antibody (PK136) or IgG2a isotype control on days 0, 2, 4, and 6 post-wounding. Wounds were harvested on days 3, 5, or 7, and NK cell depletion was shown to reduce cytokine expression in wounds (Figure 5). Statistical analysis using two-way ANOVA and Sidak’s multiple comparisons test revealed significant effects of PK136 compared to the isotype control over time (p < 0.05).
Tags: anti-mouse NK1.1 PK136 in animal model; anti-mouse NK1.1 PK136 antibody in animal model
The Mechanism of Anti-PD-L1 Antibody Efficacy against PD-L1-Negative Tumors Identifies NK Cells Expressing PD-L1 as a Cytolytic Effector
Tai LH, et al. Cancer Immunol Res. 2019 Oct;7(10):1761-1770. doi: 10.1158/2326-6066.CIR-19-0038. PMID: 31340937
The anti-NK1.1 antibody (PK136) was used to deplete NK cells in a PD-L1 knockout YAC-1 tumor model in BALB/c mice (n=5), treated with anti-PD-L1 mAb or IgG control. Flow cytometry analysis showed NK cell CD107a expression in spleen and lung, revealing impaired antitumor activity in NK-depleted or PD-L1−/− mice. The study highlighted the role of PD-L1+ NK cells as cytolytic effectors in anti-PD-L1 therapy, even in PD-L1-negative tumors.
Tags: anti-mouse NK1.1 PK136 antibody in vivo; anti-mouse NK1.1 PK136 in mouse tumor model
The Variable Genomic NK Cell Receptor Locus Is a Key Determinant of CD4+ T Cell Responses During Viral Infection
Flossdorf M, et al. Immunity. 2020 Apr 21;52(4):567-582.e7. doi: 10.1016/j.immuni.2020.02.014. PMID: 32153566
Depletion of NK cells using anti-asialo-GM1 antibody in C57BL/6.NKC129 mice infected with LCMV Armstrong resulted in a >20-fold increase in viral-specific CD4+ T cell responses. Mice (n=3–4/group) were injected with anti-asialo-GM1 or PBS on days -2 and 2 and sacrificed on day 4 post-infection. The study examined CD4+ CD16/32– CD44hi gp-61tet+ cell numbers on days 4, 5, 6, 8, and 13, highlighting the role of NK cells in modulating adaptive immune responses.
Tags: function of anti-mouse NK1.1 PK136; function of anti-mouse NK1.1 PK136 mAb
Bone marrow produces sufficient alloreactive natural killer (NK) cells in vivo to cure mice from subcutaneously and intravascularly injected 4T1 breast cancer
Laskowski P, et al. Exp Hematol. 2016 Dec;44(12):1211-1219.e3. doi: 10.1016/j.exphem.2016.09.008.
PMID: 27915436 The anti-NK1.1 antibody (PK136) was used to deplete NK cells in B6CBAF1 mice injected with 4T1 breast cancer cells, combined with chemo-irradiation on days 8 and 9. Progression-free survival (PFS) curves showed breast cancer-related deaths in untreated (n=16) or NK1.1-depleted (n=15–16) groups, with anti-NK1.1 applied throughout the experiment. The study demonstrated that bone marrow-derived alloreactive NK cells were sufficient to cure 4T1 breast cancer, prompting clinical exploration of NK-alloreactive bone marrow transplantation.
Tags: anti-NK1.1 (PK136) monoclonal antibody; mouse NK1.1 antibody (clone PK136)
了解更多抗小鼠NK1.1单克隆抗体（clone：PK136）参考文献，欢迎联系我们（咨询微信：duozhaozhao2024）。
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请记住我们的产品信息: 体内实验级重组抗小鼠NK1.1单克隆抗体(克隆号PK136)，小鼠IgG2a Kappa: PA007525.m2a Syd Labs In Vivo Grade Recombinant Anti-mouse NK1.1 Monoclonal Antibody (Clone: PK136), Mouse IgG2a Kappa。