抗人CD28单克隆抗体(克隆号CD28.2) ，体内实验级重组，小鼠IgG1 Kappa | Syd Labs PA007477.m1
体内实验级重组抗人CD28单克隆抗体，小鼠IgG1 Kappa（克隆号：CD28.2，货号：PA007477.m1）是用哺乳动物细胞生产的重组抗体，纯度>95%，适用于体外和体内研究，比如体外T细胞激活/刺激。Syd Labs PA007477.m1不变区为小鼠Mouse IgG1 Kappa (mIgG1或m1)，可与重组小鼠IgG1同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
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| 货号 | PA007477.m1 |
|---|---|
| 产品名称 | 抗人CD28单克隆抗体(克隆号CD28.2) ，体内实验级重组，小鼠IgG1 Kappa | Syd Labs PA007477.m1 |
| 英文名 | In vivo Grade Recombinant Anti-Human CD28 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: CD28.2) |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | T 细胞特异性表面糖蛋白 CD28; TP44 |
| 概述 | Syd Labs提供重组小鼠IgG1同型对照抗体，样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | CD28.2 |
| 同种型 | 小鼠IgG1 kappa |
| 应用 | ELISA、流式细胞术、中和、功能测定，如生物分析PK和ADA测定，以及用于研究受人CD28蛋白影响的生物途径的测定。 |
| 免疫源 | 抗人CD28单克隆抗体（克隆：CD28.2）是用哺乳动物细胞生产的 |
| 抗体形式 | 0.2 μM过滤溶液，1x PBS |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗人CD28单克隆抗体，小鼠IgG1 Kappa(克隆号CD28.2) 用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007477.m1 Syd Labs：体内实验级重组抗人CD28单克隆抗体（克隆号：CD28.2）是用哺乳动物细胞生产的重组抗体，可与重组小鼠IgG1同型对照抗体配套使用。样品制备条件和最佳样品稀释应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
描述
PA007477.m1: Syd Labs体内实验级重组抗人CD28单克隆抗体（克隆号CD28.2），小鼠IgG1 Kappa（In vivo Grade Recombinant Anti-Human CD28 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: CD28.2)）
抗人CD28单抗（CD28.2）参考文献:
Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells.
Blewett, M. M., et al. Sci Signal. 2016 Sep 13;9(445):rs10. doi: 10.1126/scisignal.aaf7694. PMID: 27625306
One such target, the kinase PKCθ, contains two cysteine residues that were targeted by DMF, which prevented PKCθ from interacting with the T cell costimulatory receptor CD28 and mediating full T cell activation. …DMF blocked the association of PKCθ with the costimulatory receptor CD28 by perturbing a CXXC motif in the C2 domain of this kinase. …Mutation of these DMF-sensitive cysteines also impaired PKCθ-CD28 interactions and T cell activation, designating the C2 domain of PKCθ as a key functional, electrophile-sensing module important for T cell biology. …Dimethyl fumarate (DMF) is an electrophilic drug that is used to treat autoimmune conditions, including multiple sclerosis and psoriasis. …The drug dimethyl fumarate (DMF), which is used to treat autoimmune diseases, including psoriasis and multiple sclerosis, may act by modifying cysteine residues in proteins.
Tags: anti-human CD28; activity of anti-human CD28 antibody
Ndrg1 is a T-cell clonal anergy factor negatively regulated by CD28 costimulation and interleukin-2.
Oh, Y. M., et al. Nat Commun. 2015 Oct 28;6:8698. doi: 10.1038/ncomms9698. PMID: 26507712
The most well-studied signal 2-inducing molecule on the T-cell surface is CD28. …Addition of agonistic antibody to CD28 during the induction phase prevents anergy20 and supplying CTLA4-Ig, which blocks CD28 signals during a productive stimulation, results in anergy induction. …According to the two-signal model, anergy factors downstream of TCR signalling should be inhibited by CD28 signalling, to prevent anergy induction. …First, we transfected Jurkat cells with both an Ndrg1 expression plasmid and the IL-2 promoter luciferase plasmid, and then stimulated them with anti-CD3 plus anti-CD28. …Some cells were stimulated with anti-CD28 in the presence of 1 μM of MG132 (c). Results are representative of two independent experiments.
Tags: anti-human CD28 9.3 mAb; anti-human CD28 9.3 mAb in vivo
HIV-1 Nef disrupts intracellular trafficking of major histocompatibility complex class I, CD4, CD8, and CD28 by distinct pathways that share common elements.
Leonard, J. A., et al. J Virol. 2011 Jul;85(14):6867-81. doi: 10.1128/JVI.00229-11. PMID: 21543478
The other putative targets of Nef, CD28, CD8β, CD80, CD86, and CD1d, are expressed in a variety of immune cells, including helper T lymphocytes and CTLs, as well as antigen-presenting cells (APCs), such as macrophage and dendritic cells (DC). …The CD86 cytoplasmic tail was fused to HA-HLA-A2 in a manner similar to that used for CD28. …Similarly, all of the Nef proteins downmodulated endogenous CD4 as well as A2/CD8β and A2/CD28. …Similarly, we observed AP-1 coprecipitation with A2/CD28 and A2/CD8β (Fig. 5B), although the interaction with A2/CD8β seemed weaker than that observed with HLA-A2 and A2/CD28. …Furthermore, Nef LL164,165AA is defective at downmodulating CD8β or CD28 yet retains the ability to downmodulate HLA-A2.
Tags: function of anti-human CD28 9.3; anti-human CD28 9.3 in cancer research
Paucity of CD4+ natural killer T (NKT) lymphocytes in sooty mangabeys is associated with lack of NKT cell depletion after SIV infection.
Rout, N., et al. PLoS One. 2010 Mar 24;5(3):e9787. doi: 10.1371/journal.pone.0009787. PMID: 20352088
Analysis of the surface markers CD95, CD28, CD45RA, and CXCR3 showed that the majority of the NKT clones were CD95+CD28−CD45RA− and CXCR3+ (Figure 3A–B) indicating an effector memory phenotype consistent with findings in human NKT lymphocytes. …Histogram plots gated on representative NKT clones showing surface expression of NK cell markers (CD16, CD56, NKG2D, and CD161), chemokine receptors (CCR5 and CXCR3), and memory markers (CD95, CD28, and CD45RA), and intracellular expression of cytolytic molecules (granzyme B and perforin). B) Mean % for each phenotypic marker on 5 to 71 NKT clones. …Dot plots showing CD95 vs CD28 staining on each subset of T lymphocytes (top panel) and distribution of CCR7 and CD28 on the CD95+ memory lymphocytes (bottom panel). …In comparison to memory CD8+ and DN T lymphocytes, ex vivo NKT lymphocytes consisted of a homogenous CD95+CD28+CCR7− population, consistent with a transitional effector memory phenotype.For identification of NKT cells, PBMCs were surface stained for CD3 and anti-Vα24 combined with PBS-57 loaded CD1d TM or 6B11 antibody. …Paired and unpaired t-tests were used for comparisons of grouped and ungrouped samples respectively.
Tags: bioactivity of anti-human CD28 9.3; anti-human CD28 (9.3) in vivo antibody
了解更多抗人CD28单克隆抗体（clone：CD28.2）参考文献，欢迎联系我们（咨询微信：duozhaozhao2024）。
Syd Labs抗人CD28单克隆抗体(克隆号CD28.2)，小鼠IgG1 Kappa（货号：PA007477.m1）推荐同型对照抗体:
Syd Labs提供以下体内实验级重组抗人CD28单克隆抗体：
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)
Syd Labs提供以下体内实验级重组抗小鼠CD28单克隆抗体：
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51)
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)
Syd Labs提供以下重组抗人CD28单克隆抗体（流式细胞术用）：
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry
请记住我们的产品信息: 体内实验级重组抗人CD28单克隆抗体，小鼠IgG1 Kappa (克隆号:CD28.2): PA007477.m1 Syd Labs In vivo Grade Recombinant Anti-Human CD28 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: CD28.2)。