抗小鼠CD115单克隆抗体(克隆号AFS98) ，体内实验级重组，大鼠IgG2a kappa | Syd Labs PA007394.r2a
体内实验级重组抗小鼠CD115单克隆抗体，大鼠 IgG2a kappa（克隆号：AFS98，货号：PA007394.r2a）是用哺乳动物细胞生产的重组抗体，纯度>95%，适用于体外和体内研究，比如体内巨噬细胞耗竭（清除）、体内单核细胞耗竭（清除）。Syd Labs PA007394.r2a不变区为大鼠Rat IgG2a kappa，可与重组大鼠IgG2a同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
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| 货号 | PA007394.r2a |
|---|---|
| 产品名称 | 抗小鼠CD115单克隆抗体(克隆号AFS98) ，体内实验级重组，大鼠IgG2a kappa | Syd Labs PA007394.r2a |
| 英文名 | In Vivo Grade Recombinant Anti-mouse CD115 Monoclonal Antibody, Rat IgG2a Kappa (Clone: AFS98) |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 集落刺激因子1受体，CSF1R，巨噬细胞集落刺激因素受体，M-CSFR |
| 概述 | Syd Labs提供重组大鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释应由研究人员通过实验确定。 |
| 克隆号 | AFS98 |
| 同种型 | 大鼠 IgG2a kappa |
| 应用 | ELISA、流式细胞术、中和、功能测定，如生物分析PK和ADA测定，以及用于研究受小鼠CD115蛋白影响的生物途径的测定。 |
| 免疫源 | 体内实验级重组抗小鼠CD115大鼠 IgG2a单克隆抗体在哺乳动物细胞中产生 |
| 抗体形式 | 0.2 μM过滤溶液，1x PBS |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗小鼠CD115单克隆抗体，大鼠 IgG2a kappa(克隆号AFS98) 用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007394.r2a Syd Labs提供重组大鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
描述
PA007394.r2a: Syd Labs体内实验级重组抗小鼠CD115单克隆抗体（克隆号AFS98），大鼠IgG2a Kappa（In vivo Grade Recombinant Anti-mouse CD115 Monoclonal Antibody, Rat IgG2a Kappa (Clone: AFS98)）
抗小鼠CD115单抗（AFS98）部分参考文献:
1. The M-CSF receptor in osteoclasts and beyond
Se Hwan Mun,et al.Exp Mol Med. 2020.PMCID: PMC8080670
“Colony-stimulating factor 1 receptor (CSF1R, also known as c-FMS) is a receptor tyrosine kinase. Macrophage colony-stimulating factor (M-CSF) and IL-34 are ligands of CSF1R. CSF1R-mediated signaling is crucial for the survival, function, proliferation, and differentiation of myeloid lineage cells, including osteoclasts, monocytes/macrophages, microglia, Langerhans cells in the skin, and Paneth cells in the intestine. CSF1R also plays an important role in oocytes and trophoblastic cells in the female reproductive tract and in the maintenance and maturation of neural progenitor cells. Given that CSF1R is expressed in a wide range of myeloid cells, altered CSF1R signaling is implicated in inflammatory, neoplastic, and neurodegenerative diseases. Inhibiting CSF1R signaling through an inhibitory anti-CSF1R antibody or small molecule inhibitors that target the kinase activity of CSF1R has thus been a promising therapeutic strategy for those diseases. In this review, we cover the recent progress in our understanding of the various roles of CSF1R in osteoclasts and other myeloid cells, highlighting the therapeutic applications of CSF1R inhibitors in disease conditions.”
2. Therapeutic Effects of Anti-CD115 Monoclonal Antibody in Mouse Cancer Models through Dual Inhibition of Tumor-Associated Macrophages and Osteoclasts
Laetitia Fend,et al.PLoS One. 2013.PMCID: PMC3760897
“Tumor progression is promoted by Tumor-Associated Macrophages (TAMs) and metastasis-induced bone destruction by osteoclasts. Both myeloid cell types depend on the CD115-CSF-1 pathway for their differentiation and function. We used 3 different mouse cancer models to study the effects of targeting cancer host myeloid cells with a monoclonal antibody (mAb) capable of blocking CSF-1 binding to murine CD115. In mice bearing sub-cutaneous EL4 tumors, which are CD115-negative, the anti-CD115 mAb depleted F4/80+ CD163+ M2-type TAMs and reduced tumor growth, resulting in prolonged survival. In the MMTV-PyMT mouse model, the spontaneous appearance of palpable mammary tumors was delayed when the anti-CD115 mAb was administered before malignant transition and tumors became palpable only after termination of the immunotherapy. When administered to mice already bearing established PyMT tumors, anti-CD115 treatment prolonged their survival and potentiated the effect of chemotherapy with Paclitaxel. As shown by immunohistochemistry, this therapeutic effect correlated with the depletion of F4/80+CD163+ M2-polarized TAMs. In a breast cancer model of bone metastasis, the anti-CD115 mAb potently blocked the differentiation of osteoclasts and their bone destruction activity. This resulted in the inhibition of cancer-induced weight loss. CD115 thus represents a promising target for cancer immunotherapy, since a specific blocking antibody may not only inhibit the growth of a primary tumor through TAM depletion, but also metastasis-induced bone destruction through osteoclast inhibition.”
3. An Early Microglial Response Is Needed To Efficiently Control Herpes Simplex Virus Encephalitis
Olus Uyar,et al.J Virol. 2020.PMCID: PMC7654270
“Microglia appear to be one of the principal regulators of neuroinflammation in the central nervous system (CNS). An increasing number of studies have demonstrated that the activation of microglia could result in either beneficial or detrimental effects in different CNS disorders. Hence, the role of microglia during herpes simplex virus encephalitis (HSE) has not been fully characterized. Using experimental mouse models, we showed that an early activation of the MCSF/CSF1R axis improved the outcome of the disease, possibly by inducing a proliferation of microglia. In contrast, depletion of microglia before HSV-1 infection worsened the prognosis of HSE. Thus, an early microglial response followed by sustained infiltration of monocytes and T cells into the brain seem to be key components for a better clinical outcome. These data suggest that microglia could be a potential target for immunomodulatory strategies combined with antiviral therapy to better control the outcome of this devastating disease.”
了解更多抗小鼠CD115单克隆抗体（clone：AFS98）参考文献，请查看：抗小鼠CD115抗体（克隆号AFS98）参考文献
Syd Labs抗小鼠CD115单克隆抗体(克隆号AFS98)，大鼠 IgG2a kappa（货号：PA007394.r2a）推荐同型对照抗体:
背景知识
The rat anti-mouse CD115 (CSF1R or M-CSFR) monoclonal antibody AFS98（大鼠抗小鼠CD115单克隆抗体） (rat IgG2a kappa) reacts with the mouse CD115 protein. CSF1R is found in the outer membrane of certain cell types, including microglia and macrophages. CSF1R controls the development, survival, and maintenance of microglia, and is involved in the proliferation, differentiation, and activation of macrophages.
Our recombinant AFS98 antibodies（重组抗小鼠CD115单克隆抗体） have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD115 monoclonal antibody（大鼠抗小鼠CD115抗体） (hybridoma clone name or number: AFS98).
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请记住我们的产品信息: 体内实验级重组抗小鼠CD115单克隆抗体(克隆号AFS98) ，大鼠 IgG2a kappa: PA007394.r2a Syd LabsIn Vivo Grade Recombinant Anti-mouse CD115 Monoclonal Antibody, Rat IgG2a Kappa (Clone: AFS98)。