体内实验级重组人IgG3同型对照抗体
体内实验级重组人IgG3同型对照抗体(In Vivo Grade Recombinant Human IgG3 Isotype Control Antibody)。体内实验级重组人IgG3 kappa同型对照抗体(In vivo grade recombinant human IgG3 kappa isotype control Antibody)。与经测试的人体样本的特异性结合程度低或无特异性结合。人源化可变区和人IgG3 kappa恒定区。批量订购价格更低。
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货号 | PA007160 |
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产品名称 | 体内实验级重组人IgG3同型对照抗体 |
英文名 | In Vivo Grade Recombinant Human IgG3 Isotype Control Antibody |
供货商名称 | Syd Labs, Inc. |
品牌名 | 悉得(Syd Labs) |
别称 | 重组人IgG3同型对照抗体,人IgG3阴性对照抗体,hIgG3同型对照抗体 |
概述 | 体内实验级重组人IgG3 kappa同型对照抗体(In vivo grade recombinant human IgG3 kappa isotype control Antibody)。与经测试的人体样本的特异性结合程度低或无特异性结合。人源化可变区和人IgG3 kappa恒定区。批量订购价格更低。 |
克隆号 | 4F17 |
同种型 | 人 IgG3, kappa |
应用 | 用于ELISA、蛋白质印迹 (WB)、流式细胞术(Flow)、免疫沉淀(IP)、免疫组织化学(石蜡)(IHC (P))、免疫组织化学(Frozen) (IHC (F))和体内动物模型研究的人IgG1 kappa抗体的同型匹配阴性对照。 |
免疫源 | N/A |
抗体形式 | 0.2 μM过滤溶液,1x PBS |
内毒素 | 根据 LAL 方法,≤ 1 EU每 1 mg 蛋白质。提供特级体内实验级重组人IgG3同型对照抗体(In Vivo Grade Recombinant Human IgG3 Isotype Control Antibody,内毒素≤0.05 EU/mg)。 |
纯度 | >95%(在还原条件下通过SDS-PAGE测定) |
运输 | 体内实验级重组人IgG3同型对照抗体(In Vivo Grade Recombinant Human IgG3 Isotype Control Antibody)用冰袋运输。收到后,请立即将其存放在下面建议的温度下。 |
稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 如果保存在2 至 8°C,自收到之日起可保存3个月。如果保存在-20 至 -70°C,自收到之日起可保存 12个月。 |
注意事项 | 体内实验级重组人IgG3 kappa同型对照抗体(In vivo grade recombinant human IgG3 kappa isotype control Antibody)。与经测试的人体样本的特异性结合程度低或无特异性结合。人源化可变区和人IgG3 kappa恒定区。批量订购价格更低。 |
线下下单 | 武汉多找找科技有限公司 电话: 18162581039 微信: duozhaozhao2024 Email: message@sydlabs.com或联系我们 |
描述
PA007160: 体内实验级重组人IgG3同型对照抗体(In Vivo Grade Recombinant Human IgG3 Isotype Control Antibody)
背景知识
The recombinant human IgG3 isotype control (hIgG3 kappa isotype control antibody) and mutants(重组人IgG3同型对照(hIgG3 kappa同型对照抗体)和突变体) are produced in mammalian cells (HEK293 and CHO cells). The recombinant human IgG3 isotype control(重组人IgG3同型对照) has low or no specific binding to any human sample, and humanized or completely human variable regions in addition to the human IgG3 kappa constant regions. The human IgG3 kappa isotype control and mutants(人IgG3 kappa同型对照和突变体) such as Fc-silenced can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.
The isotype controls are normally used to differentiate any non-specific background signal from the specific antibody signal, including the immunogenicity if used in animals. Most in vivo functional grade human IgG antibodies(人IgG抗体) are used in mice for in vivo studies. Besides the antibody Complementarity-Determining Regions (CDRs), two human or humanized variable regions including the one in the human or humanized IgG isotype control(人源化IgG同型对照) do not necessarily induce the same immune response in mice because in some cases, even one amino acid difference can make difference. Mouse variable regions can be used to show the potential immunogenicity induced by the human variable regions in the human or humanized IgG antibodies(人源化IgG抗体) to test. Thus, in some cases, it is better to use the mouse variable region in the human IgG isotype controls(人IgG同型对照). We also produce and sell recombinant human IgG isotype controls(重组人IgG同型对照) from the popuplar mouse hybridoma clones, MOPC21 and C1.18.4, as well as recombinant human IgG1 kappa isotype controls(重组人IgG1 kappa同型对照) against KLH, TNP, DNP, GST, HEL, His-tag, Flag, c-myc, HA, GFP, and unknown specificity.
Please contact us to ask for a quote for the Fc silenced human IgG3 isotype control mutants(Fc silenced人IgG3同型对照突变体), hIgG3 isotype control and silent Fc mutants(hIgG3同型对照和silent Fc突变体) with Avi-, His-, and Flag-tags, and biotinylated hIgG3 isotype control and Fc silent mutants(hIgG3同型对照和Fc silent突变体). A variety of conjugates (such as a fluorophore) with the human IgG3 isotype control and Fc effector-silent mutants(人IgG3同型对照和Fc effector-silent突变体) are available.
Isotype control antibodies(同型对照抗体) (negative control antibodies or reference antibodies) are frequently used in various antibody-related in vivo and in vitro experiments such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Isotype control antibodies(同型对照抗体) are used as negative controls of primary antibodies to check presence of non-specific binding to Fc receptors on the cell surface, other cellular proteins, carbohydrates, and lipids. Thus, the isotype control antibody(同型对照抗体) normally matches the host species and isotype, including light chains (such as human IgG1 kappa antibody(人IgG1 kappa抗体)), concentration, and conjugation format of the experimental primary antibody.
其它体内实验级重组IgG同型对照抗体和突变体(in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants):
体内实验级重组人IgG1同型对照抗体和突变体(In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants)
体内实验级重组人IgG2同型对照抗体(In vivo Grade Recombinant Human IgG2 Isotype Control Antibody)
体内实验级重组人IgG3同型对照抗体(In vivo Grade Recombinant Human IgG3 Isotype Control Antibody)
体内实验级重组人IgG4-S228P同型对照抗体和突变体(In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants)
体内实验级重组小鼠IgG1同型对照抗体和突变体(In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants)
体内实验级重组小鼠IgG2a同型对照抗体和突变体(In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants)
体内实验级重组小鼠IgG2b同型对照抗体和突变体(In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants)
体内实验级重组小鼠IgG2c同型对照抗体和突变体(In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants)
体内实验级重组小鼠IgG3同型对照抗体(In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody)
体内实验级重组大鼠IgG1同型对照抗体(In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody)
体内实验级重组大鼠IgG2a同型对照抗体(In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody)
体内实验级重组大鼠IgG2b同型对照抗体(In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody)
体内实验级重组大鼠IgG2c同型对照抗体(In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody)
体内实验级重组仓鼠IgG1同型对照抗体(In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody)
体内实验级重组仓鼠IgG2同型对照抗体(In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody)
体内实验级重组IgG Fc蛋白(In vivo Grade Recombinant IgG Fc Proteins):
体内实验级重组人IgG1-Fc蛋白(hIgG1)(In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1))
体内实验级重组人IgG2-Fc蛋白(hIgG2)(In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2))
体内实验级重组人IgG4 Fc蛋白(hIgG4)(In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4))
体内实验级重组小鼠IgG1-Fc蛋白(mIgG1)(In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1))
体内实验级重组小鼠IgG2a-Fc蛋白(mIgG2a)(In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a))
体内实验级重组小鼠IgG2b-Fc蛋白(mIgG2b)(In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b))
体内实验级重组大鼠IgG2a-Fc蛋白(rtIgG2a)(In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a))
体内实验级重组大鼠IgG2b-Fc蛋白(rtIgG2b)(In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b))
体内实验级重组Llama IgG2b-Fc蛋白(lIgG2b)(In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b))
体内实验级重组兔IgG Fc蛋白(rIgG)(In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG))
Fc-ELISA试剂盒和试剂(Fc ELISA Kits and Reagents):
人Fc-ELISA试剂盒(Human Fc ELISA Kit)
小鼠Fc-ELISA试剂盒(Mouse Fc ELISA Kit)
人Fc-ELISA试剂盒(Human Fc ELISA Reagent Kit)
小鼠Fc-ELISA试剂盒(Mouse Fc ELISA Reagent Kit)
蛋白质A、树脂、柱和ELISA试剂盒(Protein A, Resin, Columns, and ELISA Kits):
重组蛋白A(Recombinant Protein A)
蛋白A亲和树脂(Protein A Affinity Resin)
预填充蛋白A柱(Pre-packed Protein A Column)
蛋白A ELISA试剂盒(Protein A ELISA Kit)
请记住我们的产品信息: 体内实验级重组人IgG3同型对照抗体: PA007160 Syd Labs (In Vivo Grade Recombinant Human IgG3 Isotype Control Antibody)