重组小鼠IgG1同型对照抗体，体内实验级 | 悉得（Syd Labs） PA007126
悉得（Syd Labs）提供体内实验级重组小鼠IgG1同型对照抗体（货号：PA007126），与经测试的小鼠样本的特异性结合程度低或无特异性结合，同时还提供更多重组小鼠IgG1同型对照抗体相关产品，包括各种靶标、突变体、标签和偶联物。重组小鼠IgG1 kappa同型对照抗体和mIgG1 D265A同型对照突变体适合体外和体内研究。
- 产品参数
- 文献
- More Offers
| 货号 | PA007126 |
|---|---|
| 产品名称 | 重组小鼠IgG1同型对照抗体，体内实验级 | 悉得（Syd Labs） PA007126 |
| 英文名 | In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 别称 | 重组小鼠IgG1同型对照抗体，小鼠IgG1阴性对照抗体，mIgG1同型对照抗体 |
| 概述 | 重组小鼠IgG1 kappa同型对照抗体和mIgG1 D265A同型对照突变体适合体外和体内研究。与经测试的小鼠样本的特异性结合程度低或无特异性结合。我们还提供更多重组小鼠IgG1同型对照抗体相关产品，包括各种靶标、突变体、标签和偶联物。 |
| 克隆号 | 12B9 |
| 同种型 | 小鼠 IgG1, kappa |
| 应用 | 用于ELISA、Western Blot (WB)、流式细胞术(Flow)、免疫沉淀(IP)、免疫组织化学(石蜡)(IHC (P))、免疫组织化学(Frozen) (IHC (F))和体内动物模型研究的小鼠IgG1 kappa抗体的同型匹配阴性对照。 |
| 免疫源 | N/A |
| 抗体形式 | 0.2 μM过滤溶液，1x PBS |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质。提供特级体内实验级重组小鼠IgG1同型对照抗体（内毒素≤0.05 EU/mg)。 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组小鼠IgG1同型对照抗体用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，如果保存在2 至 8°C。 自收到之日起 3 个月，如果保存在-20 至 -70°C。 |
| 注意事项 | PA007126 悉得（Syd Labs）重组小鼠IgG1 kappa同型对照抗体和mIgG1 D265A同型对照突变体适合体外和体内研究。与经测试的小鼠样本的特异性结合程度低或无特异性结合。我们还提供更多重组小鼠IgG1同型对照抗体相关产品，包括各种靶标、突变体、标签和偶联物。 |
| 产品咨询 | 悉得（Syd Labs）在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系悉得（Syd Labs）中国代理商。 关于悉得（Syd Labs）产品如果有任何技术或其它问题，欢迎随时联系悉得（Syd Labs）国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
描述
PA007126：重组小鼠IgG1同型对照抗体，体内实验级（In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody）
背景知识
Mouse IgG1 isotype control and mutants（小鼠IgG1同型对照和突变体） (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions.
Please contact us to ask for a quote for the Fc silenced mouse IgG1 isotype control mutants（小鼠IgG1同型对照突变体）, mIgG1 isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated mIgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the mouse IgG1 isotype control and Fc-slient mutants（小鼠IgG1同种型对照和Fc slient突变体） are available.
Mouse IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a or IgG2c, IgG2b, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.
问题与答案:
Question: MOPC21 or MOPC-21 (hybridoma clone number, isotype: mouse IgG1, kappa) is widely used as a mouse IgG1 isotype control（小鼠IgG1同型对照） produced directly by hybridoma or in the recombinant formats. Is your mouse IgG1 isotype control（小鼠IgG1同型对照） also Mouse IgG1, kappa monoclonal (MOPC-21) – isotype control（小鼠IgG1，kappa单克隆（MOPC-21）同型对照）?
Answer: Similar to mouse IgG1 isotype control（小鼠IgG1同种型对照） (MOPC-21), no matter purified from hybridoma or produce by the recombinant method, our recombinant mouse IgG1 isotype controls（重组小鼠IgG1同型对照） (catalog # PA007126 and PA007135) has unknown binding specificity. But our mouse IgG1 isotype controls（小鼠IgG1同型对照） are not from the MOPC-21 hybridoma or its antibody sequences though we also produce and sell the recombinant mouse (MOPC-21) mAb IgG1 isotype control as well as Fc mutants（重组小鼠（MOPC-21）mAb-IgG1同型对照以及Fc突变体）. Please contact us and ask for the clone MOPC-21 derived recombinant mouse IgG1-k isotype controls（重组小鼠IgG1-kappa同型对照） if you are interested.
Question: What is the advantage of the recombinant antibody production method compared to the hybridoma method?
Answer: We use chemically defined medium for cell culturing to produce the recombinant antibody. There is significant advantage of the in vitro method. First, we do not use mouse ascites for antibody production. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some companies and scientists add FBS into hybridoma cell culturing medium, which results in antibody contamination. There is no such contamination in production of recombinant antibodies with the chemically defined medium. Fourth, we can easily switch the isotype with different antibody constant regions, and generate mutations such as D265A which is used in therapeutic antibody research. It is better to use the same isotype control mutation as the primary antibody in the application.
Question: What is an isotype control antibody and why is it used?
Answer: An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to differentiate, if there is any, non-specific background signal from the specific antibody signal. Mouse IgG1 kappa isotype controls（小鼠IgG1 kappa同型对照） against KLH, TNP, DNP, GST, HEL, His-tag, Flag, c-myc, HA, and GFP meet the requirements in some cases but do not in specific cases in the presence of the targets or cross-reactivity.
悉得（Syd Labs）提供以下体内实验级重组小鼠IgG1同型对照抗体和突变体（In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibodies and Mutants）:
重组小鼠IgG1同型对照抗体，体内实验级（In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody）
重组小鼠IgG1同型对照抗体，内毒素0.05 EU/mg，体内实验级（In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody, Endotoxin 0.05 EU/mg）
重组小鼠IgG1-D265A同型对照抗体，体内实验级（In Vivo Grade Recombinant Mouse IgG1-D265A Isotype Control Antibody）
重组小鼠IgG1-D265A同型对照抗体，内毒素0.05 EU/mg，体内实验级（In Vivo Grade Recombinant Mouse IgG1-D265A Isotype Control Antibody, Endotoxin 0.05 EU/mg）
悉得（Syd Labs）其它体内实验级重组IgG同型对照抗体和突变体（In vivo grade Recombinant IgG Isotype Control Antibodies and Mutants）:
重组人IgG1同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants）
重组人IgG2同型对照抗体，体内实验级（In vivo Grade Recombinant Human IgG2 Isotype Control Antibody）
重组人IgG3同型对照抗体，体内实验级（In vivo Grade Recombinant Human IgG3 Isotype Control Antibody）
重组人IgG4-S228P同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants）
重组小鼠IgG1同型对照抗体和突变体,体内实验级（In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants）
重组小鼠IgG2a同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants）
重组小鼠IgG2b同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants）
重组小鼠IgG2c同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants）
重组小鼠IgG3同型对照抗体，体内实验级（In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody）
重组大鼠IgG1同型对照抗体，体内实验级（In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody）
重组大鼠IgG2a同型对照抗体，体内实验级（In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody）
重组大鼠IgG2b同型对照抗体，体内实验级（In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody）
重组大鼠IgG2c同型对照抗体，体内实验级（In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody）
重组仓鼠IgG1同型对照抗体，体内实验级（In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody）
重组仓鼠IgG2同型对照抗体，体内实验级（In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody）
悉得（Syd Labs）体内实验级重组IgG Fc蛋白（In vivo Grade Recombinant IgG Fc Proteins）:
重组人IgG1 Fc蛋白（hIgG1），体内实验级（In vivo Grade Recombinant Human IgG1 Fc Protein （hIgG1））
重组人IgG2 Fc蛋白（hIgG2），体内实验级（In vivo Grade Recombinant Human IgG2 Fc Protein （hIgG2））
重组人IgG4 Fc蛋白（hIgG4），体内实验级（In vivo Grade Recombinant Human IgG4 Fc Protein （hIgG4））
重组小鼠IgG1 Fc蛋白（mIgG1），体内实验级（In vivo Grade Recombinant Mouse IgG1 Fc Protein（mIgG1））
重组小鼠IgG2a Fc蛋白（mIgG2a），体内实验级（In vivo Grade Recombinant Mouse IgG2a Fc Protein （mIgG2a））
重组小鼠IgG2b Fc蛋白（mIgG2b），体内实验级（In vivo Grade Recombinant Mouse IgG2b Fc Protein （mIgG2b））
重组大鼠IgG2a Fc蛋白（rtIgG2a），体内实验级（In vivo Grade Recombinant Rat IgG2a Fc Protein （rtIgG2a））
重组大鼠IgG2b Fc蛋白（rtIgG2b），体内实验级（In vivo Grade Recombinant Rat IgG2b Fc Protein （rtIgG2b））
重组羊驼IgG2b Fc蛋白（lIgG2b），体内实验级（In vivo Grade Recombinant Llama IgG2b Fc Protein（lIgG2b））
重组兔IgG Fc蛋白（rIgG），体内实验级（In vivo Grade Recombinant Rabbit IgG Fc Protein（rIgG））
请记住我们的产品信息: 体内实验级重组小鼠IgG1同型对照抗体: PA007126 Syd Labs In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody。