重组抗DYKDDDDK-Tag单克隆抗体(克隆号M2.1)，小鼠 IgG1 Kappa | Syd Labs PA000274.m1

描述

了解更多抗DYKDDDDK单抗（抗Flag单抗、抗DDDDK单抗）（clone：M2）引用文献，请查看：Flag抗体（克隆号M2）引用文献

Flag-M2抗体参考文献:

1. Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies Using Multiple Proteases and a Dual Fragmentation Scheme
Weiwei Peng,et al.J Proteome Res. 2021.PMCID: PMC8256418
“Antibody sequence information is crucial to understanding the structural basis for antigen binding and enables the use of antibodies as therapeutics and research tools. Here, we demonstrate a method for direct de novo sequencing of monoclonal IgG from the purified antibody products. The method uses a panel of multiple complementary proteases to generate suitable peptides for de novo sequencing by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a bottom-up fashion. Furthermore, we apply a dual fragmentation scheme, using both stepped high-energy collision dissociation (stepped HCD) and electron-transfer high-energy collision dissociation (EThcD), on all peptide precursors. The method achieves full sequence coverage of the monoclonal antibody herceptin, with an accuracy of 99% in the variable regions. We applied the method to sequence the widely used anti-FLAG-M2 mouse monoclonal antibody, which we successfully validated by remodeling a high-resolution crystal structure of the Fab and demonstrating binding to a FLAG-tagged target protein in Western blot analysis. The method thus offers robust and reliable sequences of monoclonal antibodies.”
2. DECODE enables high-throughput mapping of antibody epitopes at single amino acid resolution
Katsuhiko Matsumoto,et al.PLoS Biol. 2025.PMCID: PMC11756784
“Antibodies are extensively used in biomedical research, clinical fields, and disease treatment. However, to enhance the reproducibility and reliability of antibody-based experiments, it is crucial to have a detailed understanding of the antibody’s target specificity and epitope. In this study, we developed a high-throughput and precise epitope analysis method, DECODE (Decoding Epitope Composition by Optimized-mRNA-display, Data analysis, and Expression sequencing). This method allowed identifying patterns of epitopes recognized by monoclonal or polyclonal antibodies at single amino acid resolution and predicted cross-reactivity against the entire protein database. By applying the obtained epitope information, it has become possible to develop a new 3D immunostaining method that increases the penetration of antibodies deep into tissues. Furthermore, to demonstrate the applicability of DECODE to more complex blood antibodies, we performed epitope analysis using serum antibodies from mice with experimental autoimmune encephalomyelitis (EAE). As a result, we were able to successfully identify an epitope that matched the sequence of the peptide inducing the disease model without relying on existing antigen information. These results demonstrate that DECODE can provide high-quality epitope information, improve the reproducibility of antibody-dependent experiments, diagnostics and therapeutics, and contribute to discover pathogenic epitopes from antibodies in the blood.”
3. Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition
Xin-Yu Guo,et al.PLoS One. 2021.PMCID: PMC8099120
“A FLAG tag consisting of DYKDDDDK is an epitope tag that is frequently and widely used to detect recombinant proteins of interest. In this study, we performed a CRISPR-based genetic screening to identify factors involved in the detection of a FLAG-tagged misfolded model protein at the cell surface. In the screening, SLC35B2, which encodes 3’-phosphoadenosine-5’-phosphosulfate transporter 1, was identified as the candidate gene. The detection of FLAG-tagged misfolded proteins at the cell surface was significantly increased in SLC35B2-knockout cells. Furthermore, protein tyrosine sulfation mediated by tyrosyl-protein sulfotransferase 2 (TPST2) suppressed FLAG-tagged protein detection. Localization analysis of the FLAG-tagged misfolded proteins confirmed that defects in tyrosine sulfation are only responsible for enhancing anti-FLAG staining on the plasma membrane but not inducing the localization change of misfolded proteins on the plasma membrane. These results suggest that a FLAG tag on the misfolded protein would be sulfated, causing a reduced detection by the M2 anti-FLAG antibody. Attention should be required when quantifying the FLAG-tagged proteins in the secretory pathway.”

重组抗DYKDDDDK-Tag单克隆抗体，小鼠 IgG1 Kappa（克隆号M2.1，货号：PA000274.m1）

重组抗DYKDDDDK-Tag小鼠IgG1单克隆抗体（克隆号M2.1）是用哺乳动物细胞生产的重组抗体，由小鼠可变区和小鼠 IgG1 κ 不变区组成，有多种Isotype，适用于体外和体内研究，包括蛋白质纯化、WB、IP和IF等。Syd Labs 抗DYKDDDDK单抗跟FLAG M2单克隆抗体序列相同，可平替抗 FLAG M2单抗。

其它抗DYKDDDDK抗体包括:DYKDDDDK-tag单克隆抗体（DYKDDDDK-tag Monoclonal Antibody）、偶联磁珠DYKDDDDK-tag抗体（Magnetic Microbead-Conjugated DYKDDDDK-tag Monoclonal Antibody）、琼脂糖DYKDDDDK-tag单抗（Agarose-Conjugated DYKDDDDK-tag Monoclonal Antibody）。DYKDDDDK标签抗体信息可以通过duozhaozhao.com获取更多信息，包括抗dykddddk标签抗体试用装活动信息、anti-dykddddk-tag抗体WB实验数据结果信息等。

抗DYKDDDDK标签抗体相关活动信息和资讯:

新春特惠 | 悉得标签抗体买一送一
重组DYKDDDDK Tag抗体WB实验结果(1)
寻访DYKDDDDK标签抗体测评科学家
Flag标签抗体（克隆号M2）为什么最受欢迎？
Flag标签抗体使用中有哪些注意事项？
Flag标签抗体如何用于免疫细胞化学（ICC）？
Flag标签抗体在WB实验中如何表现？
Flag标签抗体如何挑选最适合的？
Flag标签抗体M1、M2、M5有何区别？
Flag标签抗体怎么用于Western Blot？
Flag标签抗体如何用于蛋白纯化？
Flag标签抗体常用类型有哪些？
Flag标签抗体是什么？

与抗DYKDDDDK标签抗体相关，我们还提供DYKDDDDK肽，产品名称：DYKDDDDK肽 Syd Labs AA0143（DYKDDDDK Peptide）

Syd Labs还提供如下常用的标签抗体:

重组抗Myc-Tag单克隆抗体(克隆号9E10)，小鼠 IgG1 Kappa
重组抗HA-Tag单克隆抗体(克隆号12CA5)，小鼠IgG2b Kappa
重组抗His-Tag单克隆抗体(克隆号3D5)，小鼠IgG2b Kappa
重组GFP-tag 单克隆抗体
重组RFP-tag 单克隆抗体
重组V5-tag 单克隆抗体

请记住我们的产品信息: 重组抗DYKDDDDK-Tag单克隆抗体（克隆号M2.1），小鼠 IgG1 Kappa: PA000274.m1 Syd Labs Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone M2.1), Mouse IgG1 Kappa。