抗人CD19单克隆抗体(克隆号SJ25C1) ，体内实验级重组，小鼠IgG1 Kappa | Syd Labs PA007270.m1
悉得（Syd Labs）体内实验级重组抗人CD19单克隆抗体，小鼠IgG1 Kappa（克隆号SJ25C1，货号：PA007270.m1），纯度>95%，适用于体外和体内研究。其不变区为小鼠Mouse IgG1 Kappa (mIgG1或m1)，可与重组小鼠IgG1同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
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| 货号 | PA007270.m1 |
|---|---|
| 产品名称 | 抗人CD19单克隆抗体(克隆号SJ25C1) ，体内实验级重组，小鼠IgG1 Kappa | Syd Labs PA007270.m1 |
| 英文名 | In Vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody（Clone SJ25C1）,Mouse IgG1 Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 别称 | B 淋巴细胞抗原 CD19，分化簇 19，B 淋巴细胞表面抗原 B4，T 细胞表面抗原 Leu-12，CVID3 |
| 概述 | 悉得（Syd Labs）提供重组小鼠IgG1同型对照抗体和重组人 IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | SJ25C1 |
| 同种型 | 小鼠 IgG1 Kappa |
| 应用 | ELISA，流式细胞术（FC），中和（neutralization），功能测定如生物分析 PK 和 ADA 测定，以及那些用于研究受人 CD19蛋白影响的生物学途径的测定。 |
| 免疫源 | 抗人 CD19单克隆抗体(克隆号: SJ25C1)是用哺乳动物细胞生产的 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，无稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗人CD19单克隆抗体(克隆号SJ25C1)，小鼠IgG1 Kappa用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 如果保存在2 至 8°C，自收到之日起可保存3个月。如果保存在-20 至 -70°C，自收到之日起可保存 12个月。 |
| 注意事项 | PA007270.m1 悉得（Syd Labs）提供重组小鼠IgG1同型对照抗体和重组人 IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | 悉得（Syd Labs）在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系悉得（Syd Labs）中国代理商。 关于悉得（Syd Labs）产品如果有任何技术或其它问题，欢迎随时联系悉得（Syd Labs）国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
描述
了解更多抗人CD19单克隆抗体（clone：SJ25C1）引用文献，请查看：抗人CD19抗体（克隆号SJ25C1）引用文献
抗人CD19单抗（SJ25C1）参考文献:
1. CD19 CAR antigen engagement mechanisms and affinity tuning
Changhao He,et al.Sci Immunol. 2023.PMCID: PMC10228544
“Chimeric antigen receptor (CAR) T cell therapy relies on T cells that are guided by synthetic receptors to target and lyse cancer cells. CARs bind to cell surface antigens through a scFv (binder), the affinity of which is central to determining CAR T cell function and therapeutic success. CAR T cells targeting CD19 were the first to achieve dramatic clinical responses in patients with relapsed/refractory B cell malignancies and to be approved by the U.S. Food and Drug Administration (FDA). We report cryo-EM structures of CD19 antigen with the binder FMC63 which is used in four FDA-approved CAR T cell therapies (Kymriah, Yescarta, Tecartus, Breyanzi), and the binder SJ25C1 which has also been used extensively in multiple clinical trials. We use these structures for molecular dynamics simulations which guide creation of lower or higher affinity binders, and ultimately produce CAR T cells endowed with distinct tumor recognition sensitivities. The CAR T cells exhibit different antigen density requirements to trigger cytolysis and differ in their propensity to prompt trogocytosis upon contacting tumor cells. Our work shows how structural information can be applied to tune CAR T cell performance to specific target antigen densities.”
2. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Andrea Cossarizza,et al.Eur J Immunol. 2021.PMCID: PMC11115438
“The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.”
3. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Andrea Cossarizza,et al.Eur J Immunol. 2019.PMCID: PMC7350392
“These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.”
Syd Labs抗人CD19单克隆抗体(克隆号SJ25C1)，小鼠IgG1 Kappa （货号：PA007270.m1）推荐同型对照抗体:
重组小鼠IgG1同型对照抗体，体内实验级（In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody）
Syd Labs提供以下体内实验级重组抗人CD19抗体:
抗人CD19单克隆抗体(克隆号: SJ25c1)（Anti-human CD19 monoclonal antibody (Clone: SJ25C1)）
抗人CD19单克隆抗体(克隆号: B43)（Anti-human CD19 monoclonal antibody (Clone: B43)）
抗人CD19单克隆抗体(克隆号: FMC63)（Anti-human CD19 monoclonal antibody (Clone: FMC63)）
Syd Labs提供以下体内实验级重组抗小鼠CD19抗体:
抗小鼠 CD19单克隆抗体(克隆号: 1D3)（Anti-mouse CD19 monoclonal antibody (Clone: 1D3)）
Syd Labs提供以下流式细胞术用重组抗人CD19抗体:
Syd Labs提供以下流式细胞术用重组抗小鼠CD19抗体:
流式细胞术用抗小鼠 CD19单克隆抗体(克隆号: 1D3)（Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry）
请记住我们的产品信息: 体内实验级重组抗人CD19单克隆抗体(克隆号SJ25C1)，小鼠IgG1 Kappa: PA007270.m1 Syd Labs In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa。