重组大鼠IgG2a同型对照抗体,体内实验级 | 悉得(Syd Labs)PA007143
重组大鼠IgG2a同型对照抗体,体内实验级(In Vivo Grade Recombinant Rat IgG2a Isotype Control Antibody,货号:PA007143)。重组大鼠IgG2a kappa同型对照抗体(Recombinant Rat IgG2a kappa isotype control Antibody)小鼠可变区和大鼠 IgG2a kappa不变区,适用于体外和体内研究。与经测试的大鼠样本的特异性结合程度低或无特异性结合。
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货号 | PA007143 |
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产品名称 | 重组大鼠IgG2a同型对照抗体,体内实验级 | 悉得(Syd Labs)PA007143 |
英文名 | In Vivo Grade Recombinant Rat IgG2a Isotype Control Antibody |
供货商名称 | Syd Labs, Inc. |
品牌名 | 悉得(Syd Labs) |
别称 | 重组大鼠IgG2a同型对照抗体,大鼠IgG2a阴性对照抗体,rtIgG2a同型对照抗体 |
概述 | 重组大鼠IgG2a kappa同型对照抗体(Recombinant rat IgG2a kappa isotype control Antibody)适合体外和体内研究。与经测试的大鼠样本的特异性结合程度低或无特异性结合。 |
克隆号 | 12B9 |
同种型 | 大鼠 IgG2a, kappa |
应用 | 用于ELISA、蛋白质印迹 (WB)、流式细胞术(Flow)、免疫沉淀(IP)、免疫组织化学(石蜡)(IHC (P))、免疫组织化学(Frozen) (IHC (F))和体内动物模型研究的大鼠IgG2a kappa抗体的同型匹配阴性对照。 |
免疫源 | N/A |
抗体形式 | 0.2 μM过滤溶液,1x PBS |
内毒素 | 根据 LAL 方法,≤ 1 EU每 1 mg 蛋白质。提供特级体内实验级重组大鼠IgG2a同型对照抗体(In Vivo Grade Recombinant Rat IgG2a Isotype Control Antibody,内毒素≤0.05 EU/mg)。 |
纯度 | >95%(在还原条件下通过SDS-PAGE测定) |
运输 | 重组大鼠IgG2a同型对照抗体,体内实验级(In Vivo Grade Recombinant Rat IgG2a Isotype Control Antibody)用冰袋运输。收到后,请立即将其存放在下面建议的温度下。 |
稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。如果保存在2 至 8°C,自收到之日起可保存1个月。如果保存在-20 至 -70°C,自收到之日起可保存 12个月。 |
注意事项 | PA007143 悉得(Syd Labs)重组大鼠IgG2a kappa同型对照抗体(Recombinant rat IgG2a kappa isotype control Antibody)适合体外和体内研究。与经测试的大鼠样本的特异性结合程度低或无特异性结合。 |
线下下单 | 武汉多找找科技有限公司 电话: 18162581039 微信: duozhaozhao2024 Email: message@sydlabs.com或联系我们 |
描述
重组大鼠IgG2a同型对照抗体,体内实验级(In Vivo Grade Recombinant Rat IgG2a Isotype Control Antibody,货号:PA007143)
背景知识
Rat IgG2a isotype control and mutants(大鼠IgG2a同型对照和突变体) (natural and engineered rtIgG2a isotype controls) are useful for functional study using animal model among a number of in vitro and in vivo research applications including ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), and Immunohistochemistry (Frozen) (IHC (F)). Rat IgG2a and IgG1 antibodies are more like mouse IgG1 than rat IgG2b antibodies. Most of rat antibodies are used to study mouse and human target proteins.
Please contact us to ask for a quote for the engineered rat IgG2a isotype control mutants(大鼠IgG2a同型对照突变体), rtIgG2a isotype control and mutants(rtIgG2a同型对照和突变体) with Avi-, His-, and Flag-tags, and biotinylated rtIgG2a isotype control and mutants(rtIgG2a同型对照和突变体). A variety of conjugates (such as dyes and fluorophores) with the rat IgG2a isotype control and Fc mutants(大鼠IgG2a同型对照和Fc突变体) are available.
Rat IgG antibodies(大鼠IgG抗体) are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a, IgG2b, and IgG2c for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.
Questions and Answers:
Question: Why do you use mouse variable regions in your recombinant rat IgG isotype controls(重组大鼠IgG同型对照)?
Answer: Most in vivo functional grade rat IgG antibodies(大鼠IgG抗体) are used to study mouse target proteins. An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to differentiate any non-specific background signal from the specific antibody signal. The use of mouse variable regions can reduce the potential immunogenicity induced by the rat variable regions in the rat IgG isotype controls(大鼠IgG同型对照) from the rat hybridoma cell lines.
Question: What is the advantage of the recombinant antibody production method compared to the hybridoma method?
Answer: We use chemically defined medium for cell culturing to produce the recombinant rat IgG isotype controls(重组大鼠IgG同型对照). There is significant advantage for the in vitro method. First, we do not use mouse ascites for antibody production. Rat hybridoma cell lines usually adapt poorly to in vitro conditions, and generate ascites in immunocompromised mice rather than rats. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some companies and scientists add FBS into hybridoma cell culturing medium, which results in antibody contamination. There is no such contamination in production of recombinant antibodies. Fourth, we can easily switch the isotype with different antibody constant regions, and generate Fc mutations such as D265A which is used in therapeutic antibody research. It is better to use the same isotype control mutation.
Question: Do you use the antibody sequences of MOPC21 (hybridoma clone number, isotype: mouse IgG1, kappa) or C1.18.4 (hybridoma clone number, isotype: mouse IgG2a, kappa) to develop your recombinant rat IgG isotype controls(重组大鼠IgG同型对照)?
Answer: Our recombinant rat IgG isotype controls(重组大鼠IgG同型对照) including the rat IgG2a isotype control(大鼠IgG2a同型对照) (catalog # PA007143) are not from MOPC21 nor C1.18.4 though we also produce and sell the MOPC21 and C1.18.4 rat IgG isotype controls(大鼠IgG同型对照). Please contact us and ask for MOPC21 and C1.18.4 derived recombinant rat IgG kappa isotype controls(重组大鼠IgG kappa同型对照) if you are interested.
悉得(Syd Labs)提供以下体内实验级重组大鼠IgG2a同型对照抗体(In Vivo Grade Recombinant Rat IgG2a Isotype Control Antibodies):
重组大鼠IgG2a同型对照抗体,体内实验级(In Vivo Grade Recombinant Rat IgG2a Isotype Control Antibody)
重组大鼠IgG2a同型对照抗体,内毒素0.05 EU/mg,体内实验级(In Vivo Grade Recombinant Rat IgG2a Isotype Control Antibody, Endotoxin 0.05 EU/mg)
悉得(Syd Labs)其它体内实验级重组IgG同型对照抗体和突变体(in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants):
重组人IgG1同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants)
重组人IgG2同型对照抗体,体内实验级(In vivo Grade Recombinant Human IgG2 Isotype Control Antibody)
重组人IgG3同型对照抗体,体内实验级(In vivo Grade Recombinant Human IgG3 Isotype Control Antibody)
重组人IgG4-S228P同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants)
重组小鼠IgG1同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants)
重组小鼠IgG2a同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants)
重组小鼠IgG2b同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants)
重组小鼠IgG2c同型对照抗体和突变体,体内实验级(In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants)
重组小鼠IgG3同型对照抗体,体内实验级(In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody)
重组大鼠IgG1同型对照抗体,体内实验级(In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody)
重组大鼠IgG2a同型对照抗体,体内实验级(In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody)
重组大鼠IgG2b同型对照抗体,体内实验级(In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody)
重组大鼠IgG2c同型对照抗体,体内实验级(In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody)
重组仓鼠IgG1同型对照抗体,体内实验级(In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody)
重组仓鼠IgG2同型对照抗体,体内实验级(In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody)
悉得(Syd Labs)体内实验级重组IgG Fc蛋白(In vivo Grade Recombinant IgG Fc Proteins):
重组人IgG1 Fc蛋白(hIgG1),体内实验级(In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1))
重组人IgG2 Fc蛋白(hIgG2),体内实验级(In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2))
重组人IgG4 Fc蛋白(hIgG4),体内实验级(In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4))
重组小鼠IgG1 Fc蛋白(mIgG1),体内实验级(In vivo Grade Recombinant Mouse IgG1 Fc Protein(mIgG1))
重组小鼠IgG2a Fc蛋白(mIgG2a),体内实验级(In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a))
重组小鼠IgG2b Fc蛋白(mIgG2b),体内实验级(In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b))
重组大鼠IgG2a Fc蛋白(rtIgG2a),体内实验级(In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a))
重组大鼠IgG2b Fc蛋白(rtIgG2b),体内实验级(In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b))
重组羊驼IgG2b Fc蛋白(lIgG2b),体内实验级(In vivo Grade Recombinant Llama IgG2b Fc Protein(lIgG2b))
重组兔IgG Fc蛋白(rIgG),体内实验级(In vivo Grade Recombinant Rabbit IgG Fc Protein(rIgG))
请记住我们的产品信息: 重组大鼠IgG2a同型对照抗体,体内实验级: PA007143 Syd Labs (In Vivo Grade Recombinant Rat IgG2a Isotype Control Antibody)。