重组抗小鼠PD-1(CD279)抗体 RMP1-14.1 | Syd Labs PA007162.r2a

描述

PA007162.r2a：Syd Labs体内实验级重组抗小鼠PD-1单克隆抗体(克隆号RMP1-14.1)，大鼠IgG2a kappa(In Vivo Grade Recombinant Anti-mouse PD-1 Monoclonal Antibody (Clone RMP1-14.1), Rat IgG2a Kappa)

Syd Labs 抗小鼠PD-1重组抗体（克隆号：RMP1-14.1，货号：PA007162.r2a）引用文献:

Spermidine potentiates anti-tumor immune responses and immunotherapy sensitivity in breast cancer
Xinyu Yang,et al.J Cancer 2025.doi:10.7150/jca.113235
“In vivo spermidine supplementing experiment：To establish a subcutaneous tumor-bearing mouse model …… Docetaxel (Sanofi Mature IP) was administered intraperitoneally at 10 mg/kg one week after tumor inoculation in mice, followed by a single intraperitoneal injection of PD-1 antibody (SYD, PA007162) at a dosage of 10 mg/kg …… The Institutional Animal Care and Use Committee at Sun Yat-Sen University granted approval for animal experiments. ”
标签：抗小鼠PD-1单抗注射剂量；RMP1-14抗体腹腔注射

抗小鼠PD-1抗体（RMP1-14）部分参考文献:

1. PD-L1/PD-1 checkpoint pathway regulates hippocampal neuronal excitability and learning and memory behavior
Junli Zhao,et al.Neuron. 2023.PMCID: PMC10529885
“Programmed death protein 1 (PD-1) and its ligand PD-L1 constitute an immune checkpoint pathway. We report that neuronal PD-1 signaling regulates learning/memory in health and disease. Mice lacking PD-1 (encoded by Pdcd1) exhibit enhanced long-term potentiation (LTP) and memory. Intraventricular administration of anti-mouse PD-1 monoclonal antibody (RMP1-14) potentiated learning and memory. Selective deletion of PD-1 in excitatory neurons (but not microglia) also enhanced LTP and memory. Traumatic brain injury (TBI) impairs learning and memory, which is rescued by Pdcd1 deletion or intraventricular PD-1 blockade. Conversely, re-expression of Pdcd1 in PD-1 deficient hippocampal neurons suppresses memory and LTP. Exogenous PD-L1 suppressed learning/memory in mice and the excitability of mouse and NHP hippocampal neurons through PD-1. Notably, neuronal activation suppressed PD-L1 secretion, and PD-L1/PD-1 signaling is distinctly regulated by learning and TBI. Thus, conditions that reduce PD-L1 levels or PD-1 signaling could promote memory in both physiological and pathological conditions.”
标签：pd 1阻断小鼠模型；抗小鼠PD-1单抗RMP1-14
2. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration
Wenji Piao,et al.Nat Commun. 2022.PMCID: PMC9023578
“Programmed death-1 (PD-1) and its ligand PD-L1 are checkpoint molecules which regulate immune responses. Little is known about their functions in T cell migration and there are contradictory data about their roles in regulatory T cell (Treg) function. Here we show activated Tregs and CD4 effector T cells (Teffs) use PD-1/PD-L1 and CD80/PD-L1, respectively, to regulate transendothelial migration across lymphatic endothelial cells (LECs). Antibody blockade of Treg PD-1, Teff CD80 (the alternative ligand for PD-L1), or LEC PD-L1 impairs Treg or Teff migration in vitro and in vivo. PD-1/PD-L1 signals through PI3K/Akt and ERK to regulate zipper junctional VE-cadherin, and through NFκB-p65 to up-regulate VCAM-1 expression on LECs. CD80/PD-L1 signaling up-regulates VCAM-1 through ERK and NFκB-p65. PD-1 and CD80 blockade reduces tumor egress of PD-1high fragile Tregs and Teffs into draining lymph nodes, respectively, and promotes tumor regression. These data provide roles for PD-L1 in cell migration and immune regulation.”
标签：体内阻断PD-1/PD-L1信号传导；PD-1阻断抗体
3. Macrophages Impair TLR9 Agonist Antitumor Activity through Interacting with the Anti-PD-1 Antibody Fc Domain
Simone Camelliti,et al.Cancers (Basel) 2021.PMCID: PMC8391891
“Simple Summary: We evaluated the contribution of macrophages to the effect of combinatorial immunotherapeutic treatments based on TLR9 stimulation (with CpG-ODNs) and PD-1 blockade in an ovarian cancer preclinical model. We observed a strong reduction in the antitumor efficacy of a TLR9 agonist upon anti-PD-1 antibody administration. Specifically, we found that TLR9-stimulated macrophages, through interacting with the fragment crystallizable (Fc) domain of the anti-PD-1 antibody, acquire an immunoregulatory phenotype leading to dampening of CpG-ODN antitumor effect. Since the stimulation of macrophage TLRs can be achieved not only by synthetic agonists but also by molecules present in the tumor microenvironment, the data we are presenting may represent another possible mechanism of anti-PD-1 antibody therapy resistance. Indeed, it is possible that when delivered as a monotherapy, anti-PD-1 antibody Fc domain may interact with macrophages in which TLR signaling has already been triggered by endogenous ligands, mirroring the biological effects described in the present study. Abstract: Background. A combination of TLR9 agonists and an anti-PD-1 antibody has been reported to be effective in immunocompetent mice but the role of innate immunity has not yet been completely elucidated. Therefore, we investigated the contribution of the innate immune system to this combinatorial immunotherapeutic regimens using an immunodeficient mouse model in which the effector functions of innate immunity can clearly emerge without any interference from T lymphocytes. Methods. Athymic mice xenografted with IGROV-1 human ovarian cells, reported to be sensitive to TLR9 agonist therapy, were treated with cytosine–guanine (CpG)-oligodeoxynucleotides (ODNs), an anti-PD-1 antibody or their combination. Results. We found that PD-1 blockade dampened CpG-ODN antitumor activity. In vitro studies indicated that the interaction between the anti-PD-1 antibody fragment crystallizable (Fc) domain and macrophage Fc receptors caused these immune cells to acquire an immunoregulatory phenotype, contributing to a decrease in the efficacy of CpG-ODNs. Accordingly, in vivo macrophage depletion abrogated the detrimental effect exerted by the anti-PD-1 antibody. Conclusion. Our data suggest that if TLR signaling is active in macrophages, coadministration of an anti-PD-1 antibody can reprogram these immune cells towards a polarization state able to negatively affect the immune response and eventually promote tumor growth.”
标签：抗PD-1抗体Fc结构；抗PD-1抗体Fc阻断

了解更多抗小鼠PD-1单克隆抗体（clone：RMP1-14）参考文献，请查看：抗小鼠PD-1单抗（克隆号RMP1-14）参考文献

Syd Labs抗小鼠PD-1重组抗体（克隆号RMP1-14.1）（货号：PA007162.r2a）推荐同型对照抗体:

重组大鼠IgG2a同型对照抗体，体内实验级（In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody）

Syd Labs相关重组IgG同型对照抗体（Recombinant IgG Reference Antibodies）:

重组小鼠IgG1同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants）
重组小鼠IgG2a同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants）
重组小鼠IgG2c同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants）

Syd Labs提供以下抗小鼠PD 1抗体（anti-mouse PD 1 antibodies）:

重组抗小鼠PD1单克隆抗体（克隆号29F.1A12.1），体内实验级（In vivo grade recombinant anti-mouse PD1 monoclonal antibodies （Clone 29F.1A12.1））
重组抗小鼠PD-1(CD279)单克隆抗体（克隆号RMP1-14.1），体内实验级（In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies （Clone RMP1-14.1））
重组鼠化抗小鼠PD 1单克隆抗体，体内实验级（In Vivo Grade Recombinant Murinized Anti-mouse PD-1 Mouse Monoclonal Antibody (Clone RMP1-14.1) 

Syd Labs提供以下体内实验级重组抗小鼠PD-1单克隆抗体(克隆号RMP1-14.1)突变体:

体内实验级重组抗小鼠PD-1单克隆抗体(克隆号RMP1-14.1)，小鼠IgG2c-LALAPG Kappa
体内实验级重组抗小鼠PD-1单克隆抗体(克隆号RMP1-14.1)，小鼠IgG2a-LALAPG Kappa
体内实验级重组抗小鼠PD-1单克隆抗体(克隆号RMP1-14.1)，小鼠IgG1-D265A Kappa

关于PA007162.r2a Syd Labs抗小鼠PD 1抗体您可能还会搜索:

PA007162.r2a Syd Labs抗小鼠PD1抗体
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PA007162.m2a RMP1-14
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背景知识

The rat anti-mouse PD-1 monoclonal antibody （大鼠抗小鼠PD-1单克隆抗体）（clone No. RMP1-14, rat IgG2a kappa）reacts with the mouse PD-1 protein (抗小鼠CD279抗体 or programmed death-1) encoded by the mouse pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD 1 antibody and the anti-mouse PD L1 antibody which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The RMP1-14 antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein.

Our recombinant RMP1-14 antibodies （重组抗小鼠CD279单克隆抗体）have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 antibody（大鼠抗小鼠PD-1抗体） （hybridoma clone name or number: RMP1-14）. The murinized anti-mouse PD-1 recombinant antibody has not only the constant regions of the mouse IgG antibodies but also the murinized variable regions.

重组抗小鼠PD 1抗体（RMP1-14 PA007162.r2a）相关问答:

Question: Which anti-mouse PD1 antibody works the best?
Answer: (by AI) There are several anti-mouse PD1 antibodies available in the market: Clones RMP1-14, 29F.1A12, and J43. All three of these antibodies are commonly used to block PD-1 signaling in vivo in murine tumor models and other mouse models. These three clones all have extensive multi-year publication records supporting them. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells). (by Syd Labs) Syd Labs offers anti-mouse PD-1 monoclonal antibodies（抗小鼠PD-1单克隆抗体） based on the sequences of clones RMP1-14 and 29F.1A12. Syd Labs provides in vivo grade recombinant antibodies including engineered antibodies for the clones RMP1-14 and 29F.1A12. Even though mouse and rat are close, rat antibodies may still induce immunogenecity in mice. Antibodies with murinized variable regions and mouse constant regions behave like humanized antibody drugs in animal models using mice. In addition, the mouse IgG2c antibody is produced in certain inbred strains such as C57BL/6, C57BL/10, SJL, and NOD, which does not express the mouse IgG2a antibody（小鼠IgG2a抗体）; the mouse IgG2a antibody（小鼠IgG2a抗体） is produced in other inbred strains such as BALB/c and Swiss Webster mice, which does not express the mouse IgG2c antibody（小鼠IgG2c抗体）. If one uses the C57BL/6 mouse strain for animal model research, it is better to use the IgG2c antibodies rather than the IgG2a antibodies. The format with the Fc silenced, Fc silent, or Fcs with silenced effector function, such as LALAPG mutation, is the most popular for anti-mouse PD1 antibodies（抗小鼠PD1抗体）（clones RMP1-14 and 29F.1A12） and anti-mouse PD-L1 antibodies（抗小鼠PD-L1抗体）（clone 10F.9G2）.

Question: Do you produce any recombinant Fc-silenced RMP1-14 antibody（重组Fc-silenced RMP1-14抗体）?
Answer: Sure, we provide various recombinant Fc silent RMP1-14 antibodies（重组Fc silent RMP1-14抗体）, such as mIgG2c LALAPG, mIgG2a LALAPG, and mIgG1 D265A. We also provide custom recombinant antibody production service to produce other engineered versions of recombinant RMP1-14 antibodies（RPM1-14单抗抗小鼠PD 1）. We have a promotion program running: We provide 1 mg PA007162.m2cLA （In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2c-LALAPG Kappa Monoclonal Antibody（体内实验级重组抗小鼠PD-1小鼠IgG2c-LALAPG Kappa单克隆抗体）（Clone RMP1-14.1）） for free in exchange of results. Please contact us to know more about the free RMP1-14 antibody.

Question: What is the difference among PA007162.r2a, PA007162.m2cLA, and PA007162.mm2cLA?
Answer: PA007162.r2a is the recombinant anti-mouse PD-1 monoclonal antibody（重组抗小鼠PD-1单克隆抗体） （rat IgG2a kappa, clone RMP1-14.1） produced in CHO cells or HEK293 cells if needed. It has the same variable region and constant region sequences as the rat anti-mouse PD-1 monoclonal antibody（大鼠抗小鼠PD-1单克隆抗体） from the hybridoma clone of RMP1-14. Rat antibodies may cause high immuogenicity in mice; thus, at least recombinant antibodies with mouse antibody constant regions should be used to replace the rat antibody constant regions. PA007162.m2cLA is the recombinant anti-mouse PD-1 antibody（重组抗小鼠PD-1抗体）（clone RMP1-14.1） whose constant regions are mouse IgG2c LALAPG kappa. We further murinize the antibody variable region sequences of PA007162.m2cLA to produce PA007162.mm2cLA.

请记住我们的产品信息: Syd Labs（货号：PA007162.r2a）重组抗小鼠PD-1单克隆抗体，大鼠IgG2a Kappa，体内实验级(克隆号RMP1-14.1): Syd Labs PA007162.r2a In Vivo Grade Recombinant Anti-mouse PD-1 Monoclonal Antibody (Clone RMP1-14.1), Rat IgG2a Kappa。