重组抗人CD8a单抗(OKT8)流式 | Syd Labs PA007418.m2a
重组抗人CD8a单克隆抗体，小鼠IgG2a Kappa（克隆号OKT8，Syd Labs货号：PA007418.m2a）是用哺乳动物细胞生产的重组抗体，适用于流式细胞术（FC）和免疫组织化学-冷冻（IHC-F）等研究，纯度>95%。其不变区为小鼠Mouse IgG2a Kappa (mIgG2a或m2a)，可与重组小鼠IgG2a同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007418.m2a |
|---|---|
| 产品名称 | 重组抗人CD8a单抗(OKT8)流式 | Syd Labs PA007418.m2a |
| 英文名 | Non-conjugated Recombinant Anti-human CD8a Monoclonal Antibody (Clone: OKT8), Mouse IgG2a Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 分化簇8，leu-2，T8 |
| 概述 | Syd Labs提供重组小鼠IgG2a同型对照抗体和重组人IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | OKT8 |
| 同种型 | 小鼠 IgG2a kappa |
| 特异性 | 该重组 OKT8 抗体与人CD8a特异性结合 |
| 免疫源 | 抗人CD8a单克隆抗体（克隆：OKT8）在哺乳动物细胞中产生 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，含0.09%叠氮化钠 |
| 偶联 | 非偶联 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 非偶联重组抗人CD8a单克隆抗体，小鼠 IgG2a Kappa(克隆号OKT8)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 12个月，保存在2 至 8°C。 请勿冻结。 |
| 注意事项 | PA007418.m2a Syd Labs提供重组小鼠IgG2a同型对照抗体和重组人IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | 流式细胞术（FC）和免疫组织化学-冷冻（IHC-F）。 |
文献
抗人CD8a单克隆抗体(克隆号OKT8)，非偶联重组，小鼠IgG2a Kappa（Non-conjugated Recombinant Anti-human CD8a Monoclonal Antibody (Clone: OKT8), Mouse IgG2a Kappa，货号：PA007418.m2a Syd Labs）
重组OKT8抗体可结合人CD8蛋白（分化群8）。CD8是一种I型跨膜糖蛋白，也是T细胞受体（TCR）的共受体。CD8共受体与TCR协同发挥作用，参与T细胞信号转导，并辅助细胞毒性T细胞与抗原之间的相互作用。CD8蛋白主要表达于细胞毒性T细胞表面，同时也表达于自然杀伤细胞（NK细胞）、皮质胸腺细胞和树突状细胞。CD8分子是细胞毒性T细胞群体的重要表面标志物。
抗人CD8a单抗(OKT8)部分参考文献:
1. T-cell receptor gene transfer exclusively to human CD8+ cells in a mouse model
Sadelain, M., et al. Blood. 2012 Nov 29;120(22):4334-41. doi: 10.1182/blood-2012-05-430330
“The OKT8 antibody (anti-human CD8a) was used in flow cytometry to confirm specific transduction of CD8+ T cells in a mouse model. OKT8 staining showed high transduction efficiency in the CD8+ population compared to CD4+ cells, with OKT8-positive cells gated for sorting, enhancing tumor killing in transduced cells.”
2. Phenotypic and functional characterization of human cytotoxic T cells lacking the OKT8 antigen
Landay, A., et al. J Immunol. 1983 Aug;131(2):1016-9.
“The OKT8 antibody (anti-human CD8a) was used in flow cytometry to identify cytotoxic T cells lacking the OKT8 antigen. OKT8-negative CTL clones exhibited normal cytolytic activity, indicating that the absence of OKT8 did not impair function, with OKT8 serving as a marker for conventional CTL.”
3. A human NK and K cell subset shares with cytotoxic T cells expression of the antigen recognized by antibody OKT8
Perussia, B., et al. J Immunol. 1983 Jul;131(1):223-31.
“The OKT8 antibody (anti-human CD8a) identified a subset of NK and K cells expressing the OKT8 antigen, detected in 20-30% of NK cells via flow cytometry. OKT8-positive NK cells showed cytotoxic activity similar to T cells, and OKT8 was used to isolate this subset for further analysis.”
4. Flow cytometric studies of the binding of monoclonal antibodies (OKT3, OKT4 and OKT8) to human T cells
Boyer, K. M., et al. J Immunol Methods. 1988 Dec 9;115(1):55-62. doi: 10.1016/0022-1759(88)90310-9
“The OKT8 antibody (anti-human CD8a) was analyzed for binding to CD8+ T cells via flow cytometry at various temperatures. OKT8 showed endothermic binding characteristics, with increased fluorescence intensity at higher temperatures, and its affinity was calculated from flow cytometry data for cytometric detection.”
5. Interactions of lectins and monoclonal antibodies with human T-cell subsets defined by OKT4 and OKT8 antibodies
Reinherz, E. L., et al. J Immunol. 1983 Jan;130(1):67-72.
“The OKT8 antibody (anti-human CD8a) was used in flow cytometry to define cytotoxic T cells, with lectin treatment enhancing OKT8 staining. OKT8-defined subsets showed differential lectin responses, and binding interactions were quantified by mean fluorescence intensity in flow cytometry assays.”