D-荧光素钾盐进口品牌Syd Labs为什么值得信赖？

D-荧光素钾盐进口品牌Syd Labs为什么值得信赖？这篇文章解释了原因

美国进口品牌Syd Labs 的D-荧光素钾盐（D-Luciferin Potassium Salt,CAS号:115144-35-9）为什么值得客户信赖？Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170）是由美国Syd Labs公司自主研发、久经全球市场考验的优质产品。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）被众多欧美大学core facilities 购买、使用和复购，在国际市场上赢得了良好的口碑。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）为什么能够取得这样的好成绩？原因主要在于，Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）相较于同类竞品在价格和质量方面存在很大优势。

Syd Labs D-荧光素钾盐（货号：MB000102-R70170或MB102-R70170）被许多国际知名学术期刊收录，充分体现了其在科研领域中不俗的实用价值。以下三篇文献展示了Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）在具体科研领域的部分应用：

28.

Aging of mice is associated with p16 (Ink4a)-and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cells

Brandon M Hall, Vitaly Balan, Anatoli S Gleiberman, Evguenia Strom, Peter Krasnov, Lauren P Virtuoso, Elena Rydkina, Slavoljub Vujcic, Karina Balan, Ilya Gitlin, Katerina Leonova, Alexander Polinsky, Olga B Chernova, Andrei V Gudkov

Aging (Albany NY). 2016 Jul;8(7):1294-315. doi: 10.18632/aging.100991.

Mice were injected intraperitoneally with a 200 μl solution of 15 mg/mL D-luciferin potassium salt (Syd Labs; Boston, MA) in D-PBS without calcium and magnesium. At 10 minutes post-injection, isoflurane-anesthetized mice were placed into the IVIS Spectrum in vivo bioluminescent imaging system (PerkinElmer; Waltham, MA) for detection of luciferase activity (60-second exposure). Bioluminescence in p16LUC mice was quantified as total flux (p/s) of luminescent signal from the abdomen using via Living Image® software.

29.

Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids

Junchen Liu, Ransome van der Hoeven, Walaa E Kattan, Jeffrey T Chang, Dina Montufar-Solis, Wei Chen, Maurice Wong, Yong Zhou, Carlito B Lebrilla, John F Hancock

Nat Commun. 2023 Jan 28;14(1):465. doi: 10.1038/s41467-023-36128-5.

Murine pancreatic adenocarcinoma cells (KPC cells) derived from KRASG12D; Trp53R172H; Pdx1-Cre (KPC) mice of C57BL/6 background were provided by Dr. Jennifer Bailey, McGovern Medical School, Houston, TX. 0.5 × 106 luciferase-expressing KPC cells that express non-targeting control (sgCtrl) or UGCG targeted (sgUGCG) CRISPR-cas9 vectors, were suspended in 50 μl saline and then injected into the pancreas of C57BL/6 mice by laparotomy. Mice were randomized into two groups: sgCtrl and sgUGCG with 10 mice for each group. 150 mg/kg D-luciferin (# MB102, Syd Labs) was intraperitoneally administrated to each mouse and allowed to spread for 2 min followed by 3 min of anesthesia. Mice were then subjected to luminescence imaging using IVIS Lumina XR Imaging System (Caliper Life Sciences). Mice were imaged 1–2 times per week for 3–4 weeks until the first mouse died. A post-implantation survival curve for all mice was subsequently recorded.

30.

A Case Study of Chimeric Antigen Receptor T Cell Function: Donor Therapeutic Differences in Activity and Modulation with Verteporfin

Jiyong Liang, Dexing Fang, Joy Gumin, Hinda Najem, Moloud Sooreshjani, Renduo Song, Aria Sabbagh, Ling-Yuan Kong, Joseph Duffy, Irina V Balyasnikova, Seth M Pollack, Vinay K Puduvalli, Amy B Heimberger

Cancers (Basel). 2023 Feb 8;15(4):1085. doi: 10.3390/cancers15041085.

Animal experiments were carried out according to regulations from the Institutional Animal Care and Use Committee (IACUC) at MD Anderson Cancer Center (ACUF 00001544-RN00). Both female and male NOD.Cg-PrkdcscidIL2Rγtm1Wjl/Sz (NSG, Jackson Laboratory, Bar Harbor, ME) mice aged 6–8 weeks were anesthetized by intraperitoneal injection using a cocktail of 10 mg/mL ketamine and 0.5 mg/mL xylazine at a dose of 0.1 mL/10 g. A guide screw was surgically implanted 2.5 mm to the right of the coronal suture and 1 mm posterior to the bregma at a depth of 3 mm. Two weeks after surgery, intracranial tumors were established by implantation of 250,000 U87-EGFRvIII-Zeomycin in 5 μL PBS through the guide screw. The CAR infiltration in the tumor was serially imaged using Xenogen IVIS Spectrum (Caliper Life Sciences, Hopkinton, MA, USA) 10 min after intraperitoneal injection of 3 mg D-luciferin potassium salt (cat. # MB000102-R70170, Syd Labs, Natick, MA). CAR flux (photons/s/cm2/steradian) was measured using Living Image software (version 2.50, Caliper Life Sciences, Hopkinton, MA, USA) in a delineated region encompassing the entire cranium. Mice were sorted into treatment groups 4 days after tumor implantation. The mice were treated on day 5 with 4 × 106 EGFRvIII CAR T cells in 5 μL of PBS administered intracranially through the guide screw and treated per the designated schemas. Mice were treated with verteporfin (10 mg/Kg) via intraperitoneal injection. Mice were sacrificed when they displayed progressive weight loss of >25%, rapid weight loss of >10% within 48 h, hind limb paralysis, or any two of the following clinical symptoms of illness: ataxia, hunched posture, or irregular respiration rate.