体内研究D-荧光素钾盐活体成像怎么做？

体内研究D-荧光素钾盐活体成像怎么做？看完这些文献就懂了

体内研究D-荧光素钾盐（D-Luciferin Potassium Salt,CAS号:115144-35-9）活体成像怎么做？Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号MB000102-R70170或MB102-R70170），作为全球市场验证的动物体内实验产品，以卓越的品质与颇具竞争力的价格脱颖而出。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）现已登陆中国市场，旨在为中国高校的动物实验中心注入全新科研动力。

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170）凭借其卓越性能，已获得欧美多所大学core facilities的广泛好评。作为美国Syd Labs公司的特色产品之一，Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）不仅在众多欧美实验室中被用于动物体内实验，而且被许多国际知名学术期刊大量收录，这充分说明Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）在科研领域具有高度的实用价值。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）被以下两篇文献引用：

53.

Radiation Synergizes with IL2/IL15 Stimulation to Enhance Innate Immune Activation and Antitumor Immunity

Xuefeng Li,et al.

Mol Cancer Ther. 2024 Mar 4;23(3):330-342. doi: 10.1158/1535-7163.MCT-23-0236.

At indicated time after tumor inoculation, mice were intraperitoneally injected with 1.5 mg D-luciferin (Syd Labs) per 10 g body weight……

54.

A drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent local relapse and treat established brain metastasis

Lucía Zhu,et al.

EMBO Mol Med. 2022 Mar 7;14(3):e14552. doi: 10.15252/emmm.202114552. Epub 2022 Feb 17.

All animal experiments were performed in accordance with a protocol approved by the CNIO (IACUC.030-2015), Instituto de Salud Carlos III (CBA35_2015-v2) and Comunidad de Madrid Institutional Animal Care and Use Committee (PROEX250/15 and PROEX135/19). Athymic nu/nu (Harlan) 4-10 weeks of age were used. Brain colonization assays were performed by injecting 100 μL PBS into the left ventricle containing 100,000 cancer cells or 2 μL RPMI1640 intracranially (the right frontal cortex, approximately 1.5 mm lateral and 1 mm caudal flow bregma, and to a depth of 1 mm) containing 100,000 cancer cells by using a gas-tight Hamilton syringe and a stereotactic apparatus. Brain colonization was analyzed in vivo and ex vivo by BLI. Anesthetized mice (isoflurane) were injected retro-orbitally with D-luciferin (150 mg/kg; Syd Labs) and imaged with an IVIS machine (Perkin Elmer). Bioluminescence analysis was performed using Living Image software, version 4.5. Brain tumor resection was performed by adapting previously described procedures (Morrissy et al., 2016). In brief, after exposing the skull, a craniotomy is performed surrounding the tumor area, which is visualized by GFP. The skull and the dura are lifted with micro-dissecting forceps, the bulk of the tumor is then removed using a microcurette guided by GFP. When hemostasis is obtained, the surgical wound is sutured using interrupted stitching with absorbable sutures. Animals receive meloxicam at 5 mg/kg once per day during 72h and dexamethasone at 13 mg/kg once per day during 48h to contain brain edema. DEBIO-0932 was administered by oral gavage (160 mg/kg) for 3 weeks, daily during the first week and once every 48h during the second week, starting 7 or 14 days after intracardiac inoculation of cancer cells for preventive or interventive therapy, respectively. For preventive therapy of relapse after neurosurgery, DEBIO0932 was administered by oral gavage (160 mg/kg) for 5-6 weeks, starting 3 days after neurosurgery.