D-荧光素钾盐（D-Luciferin Potassium Salt）怎么发光？

D-荧光素钾盐怎么发光？看这些文献就懂了

D-荧光素钾盐（D-Luciferin Potassium Salt,CAS号:115144-35-9）怎么发光？Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号MB000102-R70170或MB102-R70170），是一款由美国Syd Labs公司自主研发并长期经受国际市场考验、主要用于动物体内实验的优质产品。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）现已登陆中国市场，旨在为中国高校的动物实验中心和实验室注入全新科研动力。

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170），凭借其杰出表现，已赢得欧美众多大学core facilities的高度评价。作为美国Syd Labs公司的特色产品之一，Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）不仅在众多欧美实验室中被用于动物体内实验，而且被许多国际知名学术期刊大量收录，这充分说明Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）在科研领域具有高度的实用价值。

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170）在以下三篇文献中部分展现了其在生物科学具体研究领域的应用价值：

45.

Induction of a tier-1-like phenotype in diverse tier-2 isolates by agents that guide HIV-1 Env to perturbation-sensitive, nonnative states

Jacklyn Johnson,et al.

J Virol. 2017 Jul 12;91(15):e00174-17. doi: 10.1128/JVI.00174-17. Print 2017 Aug 1.

Canis familiaris thymus (Cf2Th) normal cells (obtained from the NIH AIDS Reagent Program) expressing CD4 and CCR5 (Cf2Th CD4+ CCR5+) or only CCR5 (Cf2Th CD4− CCR5+) were used as target cells for measuring infection. Approximately 5 h before infection, the target cells were detached from culture plates using PBS containing 7.5 mM EDTA and seeded in 96- or 384-well luminometer-compatible plates (at a density of 4.5 × 103 or 1.8 × 103 cells per well, respectively). For neutralization assays, virus preparations were incubated on ice or at 37°C in the absence or presence of the inhibitor for 2 h. Unless otherwise indicated, all neutralization assays were conducted using the following concentrations of inhibitors: PS(A) and PS(B), 1:50 dilution; PS(C) and PS(D), 1:200 dilution; 447-52d and 19b, 2 μg/ml; 10E8 and VRC01, 5 μg/ml; 17b, 3BC176, and 48d, 8 μg/ml; 2G12 and b12, 0.5 μg/ml; T20, 150 nM. Samples were then added to CD4+ CCR5+ cells, which were incubated for 3 days to allow infection. In experiments that examined the effect of urea on infection, cells were incubated with inhibitor (in the absence or presence of urea) for 6 h at 37°C. The cells were then washed and further cultured for 3 days. To measure infection, the medium was removed, and the cells were lysed with passive lysis buffer (Promega) and subjected to three freeze-thaw cycles. To measure luciferase activity, 100 μl of luciferin buffer (15 mM MgSO4, 15 mM KPO4 [pH 7.8], 1 mM ATP, and 1 mM dithiothreitol) and 50 μl of 1 mM d-luciferin potassium salt (Syd Laboratories, MA) were added to each sample in 96-well plates (30 μl and 15 μl for samples in 384-well plates). Luminescence was recorded using a Synergy H1 microplate reader (BioTek Instruments). The synergistic effects between two- and three-treatment combinations were calculated as described by equation 1 and equation 2, respectively.

46.

Properties of HIV-1 env and human seminal fluid that determine virus inhibition by antibodies and microbicides

Johnson, Jacklyn,et al.

Summer 2019；DOI: 10.17077/etd.c4n5-okpp

Canis familiaris thymus normal (Cf2Th) cells (obtained from the NIH AIDS Reagent Program) expressing CD4 and CCR5 (Cf2Th CD4+CCR5+) or only CCR5 (Cf2Th CD4‒CCR5+)were used as target cells for measuring infection. Approximately 5 h before infection, target cells were detached from culture plates using PBS containing 7.5 mM EDTA and seeded in 96- or 384-well luminometer-compatible plates (at a density of 4.5 or 1.8 × 103 cells per well, respectively). For neutralization assays, virus preparations were incubated on ice or at 37°C in the absence or presence of the inhibitor for two h. Unless indicated otherwise, all neutralization assays were conducted using the following concentrations of inhibitors: PS(A) and PS(B) 1:50 dilution, PS(C) and PS(D) at a 1:200 dilution; 447-52d and 19b at 2μg/ml; 10E8 and VRC01 at 5μg/ml; 17b, 3BC176 and at 48d 8μg/ml; 2G12 and b12 at 0.5μg/ml; T20 at 150nM. Samples were then added to CD4+CCR5+cells, which were incubated for three days to allow infection. In experiments that examined the effect of urea on infection, cells were incubated with inhibitor (in the absence or presence of urea) for six h 6 h at 37°C. Cells were then washed and further cultured for three days. To measure infection medium was removed, cells were lysed with passive lysis buffer (Promega) and subjected to three freeze-thaw cycles. To measure luciferase activity, 100 µl of luciferin buffer(15 mM MgSO4, 15 mM KPO4 [pH 7.8], 1 mM ATP, and 1 mM dithiothreitol) and 50 µl of 1 mM D-luciferin potassium salt (Syd Labs, MA) were added to each sample in 96-well plates (30 µl and 15 µl for samples in 384-well plates, respectively). Luminescence was recorded using a Synergy H1 microplate reader (BioTek Instruments). The synergistic effects between two- and three-treatment combinations were calculated as described by Equation 1 and Equation 2, respectively.

47.

Accurate predictions of population-level changes in sequence and structural properties of HIV-1 Env using a volatility-controlled diffusion model

Orlando DeLeon,et al.

PLoS Biol. 2017 Apr 6;15(4):e2001549. doi: 10.1371/journal.pbio.2001549. eCollection 2017 Apr.

Canis familiaris thymus normal (Cf2Th) cells (obtained from the NIH AIDS Reagent Program) expressing CD4 and CCR5 (Cf2Th CD4+CCR5+) or CD4 and CXCR4 (Cf2Th CD4+CXCR4+) were used as target cells for measuring infection. Approximately 5 h before infection, cells were detached from culture plates using PBS supplemented with 7.5 mM EDTA and were seeded in 96- or 384-well, luminometer-compatible plates (at a density of 14 or 4.5 × 103 cells per well, respectively). Viruses were then added to the cells and further incubated for 3 d, at which time the medium was removed; cells were lysed with passive lysis buffer (Promega) and subjected to three freeze–thaw cycles. To measure luciferase activity, 100 μl of luciferin buffer (15 mM MgSO4, 15 mM KPO4 [pH 7.8], 1 mM ATP, and 1 mM dithiothreitol) and 50 μl of 1 mM D-luciferin potassium salt (Syd Labs, MA) were added to each sample in 96-well plates (30 μl and 15 μl, respectively, for samples in 384-well plates). Luminescence was recorded using a Synergy H1 microplate reader (BioTek Instruments).