Recombinant Human FGF Acidic 136 (aFGF)
Syd Labs Recombinant Human FGF Acidic 136 (aFGF) BP000029-GD3, The FGF acidic (aFGF, FGF-1, FGF1, Acidic Fibroblast Growth Factor, aFGF, Heparin-Binding Growth Factor 1, HBGF-1, Fgf1, Fgf-1, Fgfa) activity is measured in a NIH/3T3 cell proliferation assay. The ED50 for this effect is typically 0.1-0.3 ng/ml in the presence of 10 ng/ml of heparin.
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| 货号 | BP000029-GD3 |
|---|---|
| 产品名称 | Recombinant Human FGF Acidic 136 (aFGF) |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 别称 | Acidic Fibroblast Growth Factor, FGF Acidic, Acidic FGF, ECGF, HBGF-1 |
| 来源 | E. coli-derived. |
| 形式 | Lyophilized from a 0.2 um filtered solution in PBS, pH 7.2. |
| 纯度 | > 95% by SDS-PAGE under reducing conditions. |
| 内毒素 | <1.0 EU per 1 ug of the protein by the LAL method. |
| 运输 | The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below. |
| 注意事项 | Conditions of optimal FGF-1 / aFGF / FGF1 Protein performance should be determined experimentally by the investigator. |
| 线下下单 | 电话: 18162660842微信: duozhaozhao_2017Email: message@sydlabs.comOr 联系我们 |
描述
BP000029-GD3: Recombinant Human FGF Acidic 136 (aFGF)
Background
FGF acidic, also known as aFGF/FGF-1/FGF1, ECGF, and HBGF 1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic protein (aFGF/FGF-1/FGF1) is a potent growth factor for fibroblasts and endothelial cells (1). FGF acidic protein (aFGF/FGF-1/FGF1) is involved in wound repair, angiogenesis, and development (1). FGF acidic protein (aFGF/FGF-1/FGF1) is secreted from cells via an endoplasmic reticulum/Golgi independent mechanism (1,2). The ability of FGF acidic protein (aFGF/FGF-1/FGF1) to bind to heparin sulfate is required for its ability to interact with FGF receptors and induce signaling (1-4). There are four distinct FGF receptors and each has multiple splice variants (1,3). FGF acidic protein (aFGF/FGF-1/FGF1) binds with high affinity to many, but not all, FGFRs (1). Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLC /PKC, and PI3K/Akt pathways (1).
Source: E. coli-derived.
N-terminal sequence analysis: Gly-Leu18-Asp155, with an N-terminal Gly
Predicted molecular mass: 15.5 kDa
The FGF acidic protein (aFGF/FGF-1/FGF1) activity is measured in a NIH/3T3 cell proliferation assay. The ED50 for this effect is typically 0.1-0.3 ng/ml in the presence of 10 ug/ml of heparin.
Endotoxin: <1.0 EU per 1 ug of the protein by the LAL method.
Purity: > 95% by SDS-PAGE under reducing conditions and visualized by silver stain.
Formulation: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.2.
Reconstitution: Reconstitute at 10 mg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping: The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Please remember our product information: Recombinant Human FGF Acidic 136 (aFGF): BP000029-GD3 Syd Labs