Human Activin A Protein No Tag | Syd Labs BP003826

Human Activin A Protein BP003826 Syd Labs

Human Activin A Protein No Tag | Syd Labs BP003826

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(悉得)Syd Labs 货号:BP003826 Human Activin A Protein with No Tag (Human Activin A 蛋白 No Tag ) is expressed from HEK293 without tag.It contains Gly311-Ser426.

SKU: BP003826 分类: ,

产品参数

货号 BP003826
产品名称Human Activin A Protein No Tag | Syd Labs BP003826
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
物种 Human
来源 The recombinant Human Activin A Protein, (amino acids Gly311-Ser426) fused with No tag at the terminus, was produced in HEK 293 cells.
产品标签 No Tag
生物素化 no
确定序列 Gly311-Ser426
分子量 The recombinant Human Activin A protein with no tag has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human Activin A protein with no tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human Activin RIIB protein with the His tag with the EC50 of 21.4 ng/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human Activin A Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human Activin A Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Human Activin A Protein performance should be determined experimentally by the investigator.
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文献

BP003826: Human Activin A Protein with No Tag (Human Activin A 蛋白 No Tag)

Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.
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