Mouse VEGF R3/FLT4 Protein C-hFc Tag | Syd Labs BP003315
(悉得）Syd Labs 货号：BP003315 Mouse VEGF R3/FLT4 Protein with the C-hFc Tag (小鼠 VEGF R3/FLT4 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Tyr25-Glu775.
产品参数
| 货号 | BP003315 |
|---|---|
| 产品名称 | Mouse VEGF R3/FLT4 Protein C-hFc Tag | Syd Labs BP003315 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Mouse |
| 来源 | The recombinant Mouse VEGF R3/FLT4 Protein, (amino acids Tyr25-Glu775) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | P35917 |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Tyr25-Glu775 |
| 分子量 | The recombinant Mouse VEGF R3/FLT4 protein with the hFc tag at the C-terminus has a predicted MW of 111.69 kDa. Due to glycosylation, the protein migrates to 115-160 kDa based on Bis-Tris PAGE result. |
| 活性 | Immobilized recombinant Mouse VEGF R3 protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human VEGF-C protein with the C-His tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test). The affinity constant of 12.93 pM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Mouse VEGF R3/FLT4 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Mouse VEGF R3/FLT4 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Mouse VEGF R3/FLT4 Protein performance should be determined experimentally by the investigator. |
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文献
BP003315: Mouse VEGF R3/FLT4 Protein with the C-hFc Tag (小鼠 VEGF R3/FLT4 蛋白 C-hFc 标签)
Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.
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