Human VEGF121 Protein C-His-Avi Tag | Syd Labs BP004957
(悉得）Syd Labs 货号：BP004957 Human VEGF121 Protein with the C-His-Avi Tag (人 VEGF121 蛋白 c-his-avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg147.
产品参数
| 货号 | BP004957 |
|---|---|
| 产品名称 | Human VEGF121 Protein C-His-Avi Tag | Syd Labs BP004957 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609 |
| 物种 | Human |
| 来源 | The recombinant Human VEGF121 Protein, (amino acids Ala27-Arg147) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | P15692-9 |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Ala27-Arg147 |
| 分子量 | The recombinant Human VEGF121 protein with the His-Avi tag at the C-terminus has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
| 活性 | Immobilized recombinant Human VEGF121 protein with the C-His-Avi tag at 2 ug/ml (100 ul/Well). Dose response curve for the recombinant Biotinylated Human VEGFR1 protein with the C-His tag with the EC50 of 42 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS,250mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human VEGF121 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human VEGF121 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human VEGF121 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004957: Human VEGF121 Protein with the C-His-Avi Tag (人 VEGF121 蛋白 c-his-avi 标签)
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
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