Human G-CSF Protein N-hFc Tag | Syd Labs BP003210
(悉得)Syd Labs 货号:BP003210 Human G-CSF Protein with the N-hFc Tag (人 G-CSF 蛋白 N-hFc 标签) is expressed from HEK293 with hFc tag at the N-terminal.It contains Thr31-Pro204.
产品参数
| 货号 | BP003210 |
|---|---|
| 产品名称 | Human G-CSF Protein N-hFc Tag | Syd Labs BP003210 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Human |
| 来源 | The recombinant Human G-CSF Protein, (amino acids Thr31-Pro204) fused with a hFc tag at the N-terminus, was produced in HEK 293 cells. |
| 产品标签 | N-hFc |
| 生物素化 | no |
| 确定序列 | Thr31-Pro204 |
| 分子量 | The recombinant Human G-CSF protein with the hFc tag at the N-terminus has a predicted MW of 44.20 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human G-CSF R protein with the N-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human G-CSF protein with the N-hFc tag with the EC50 of 6.0 ng/ml determined by ELISA. |
| Form | Liquid |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human G-CSF Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human G-CSF Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 注意事项 | Conditions of optimal recombinant Human G-CSF Protein performance should be determined experimentally by the investigator |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品,不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题,欢迎随时联系Syd Labs国内市场推广合作伙伴:武汉多找找科技有限公司,企业微信:duozhaozhao2024 联系电话:18162581039(龙经理) |
文献
BP003210: Human G-CSF Protein with the N-hFc Tag (人 G-CSF 蛋白 N-hFc 标签)
Recombinant Human G-CSF Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Thr31-Pro204.
Granulocyte colony-stimulating factor (G-CSF) is an hemopoietic growth factor produced by fibroblasts, monocytes and endothelial cells. G-CSF is expressed by numerous cell types including neuronal, immune and endothelial cells. G-CSF is released in autocrine manner and binds to its receptor G-CSF-R which further activates numerous signaling transduction pathways including PI3K/AKT, JAK/STAT and MAP kinase, and thereby promote neuronal survival, proliferation, differentiation, mobilization of hematopoietic stem and progenitor cells.
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