Human TIE2 Protein C-His Tag | Syd Labs BP003415

Human TIE2 Protein BP003415 Syd Labs

Human TIE2 Protein C-His Tag | Syd Labs BP003415

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(悉得)Syd Labs 货号:BP003415 Human TIE2 Protein with the C-His Tag (人 TIE2 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Ala23-Leu748.

SKU: BP003415 分类: ,

产品参数

货号 BP003415
产品名称Human TIE2 Protein C-His Tag | Syd Labs BP003415
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
物种 Human
来源 The recombinant Human TIE2 Protein, (amino acids Ala23-Leu748) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Accession AAA61139.1
产品标签 C-His
生物素化 no
确定序列 Ala23-Leu748
分子量 The recombinant Human TIE2 protein with the His tag at the C-terminus has a predicted MW of 82.09 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
活性 The affinity constant of 2.38 uM as determined in SPR assay (Biacore T200).
Form Liquid
形式 Supplied as 0.22μm filtered solution in PBS (pH 7.4).
制剂 It is recommended to reconstitute the lyophilized recombinant Human TIE2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human TIE2 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human TIE2 Protein performance should be determined experimentally by the investigator.
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文献

BP003415: Human TIE2 Protein with the C-His Tag (人 TIE2 蛋白 C-His 标签)

Angiopoietin-1 (Ang-1) is the primary agonist for Tie2 tyrosine kinase receptor (Tie2), and the effect of Ang-1-Tie2 signalling is context-dependent. Deficiency in either Ang-1 or Tie2 protein leads to severe microvascular defects and subsequent embryonic lethality in murine model. Tie2 receptors are expressed in several cell types, including endothelial cells, smooth muscle cells, fibroblasts, epithelial cells, monocytes, neutrophils, eosinophils and glial cells.
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