Human Her2/ErbB2 Protein C-His-Avi Tag | Syd Labs BP005395
(悉得)Syd Labs 货号:BP005395 Human Her2/ErbB2 Protein with the C-His-Avi Tag (人 Her2/ErbB2 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr23-Thr652.
产品参数
| 货号 | BP005395 |
|---|---|
| 产品名称 | Human Her2/ErbB2 Protein C-His-Avi Tag | Syd Labs BP005395 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; NGL; p185erbB2; TKR1; ERBB2; HER-2/neu; NEU; ENV; ENVW; ERVWE1; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV |
| 物种 | Human |
| 来源 | The recombinant Human Her2/ErbB2 Protein, (amino acids Thr23-Thr652) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Thr23-Thr652 |
| 分子量 | The recombinant Human Her2/ErbB2 protein with the His-Avi tag at the C-terminus has a predicted MW of 72.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human Her2 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Her2 Antibody protein with the C-hFc tag with the EC50 of 7.4 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Her2/ErbB2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human Her2/ErbB2 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human Her2/ErbB2 Protein performance should be determined experimentally by the investigator. |
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文献
BP005395: Human Her2/ErbB2 Protein with the C-His-Avi Tag (人 Her2/ErbB2 蛋白 C-His-Avi 标签)
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane.
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