Biotinylated Recombinant SARS Spike S1-Fc-Avi Protein

Biotinylated Recombinant SARS Spike S1-Fc-Avi Protein: BP003074 Syd Labs

Biotinylated Recombinant SARS Spike S1-Fc-Avi Protein

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Syd Labs Biotinylated Recombinant SARS Spike S1-Fc-Avi Protein BP003074, Biotinylated recombinant SARS-CoV-2 Spike S1 protein (S1 subunit, Gln14-Arg683) expressed in HEK 293 cells. Immobilized biotinylated recombinant SARS-CoV-2 Spike protein, S1 subunit. Dose response curve for rhACE2-Fc fusion protein with the EC50 of 81 ng/ml determined by ELISA.

SKU: BP003074 分类: ,
货号 BP003074
产品名称Biotinylated Recombinant SARS Spike S1-Fc-Avi Protein
供货商名称 Syd Labs, Inc.
品牌名 悉得(Syd Labs)
别称 2019-nCoV S Protein S1 Subunit, COVID-19 Virus Spike Protein S1 Subunit
来源 recombinant SARS-CoV-2 Spike S1 protein (S1 subunit, Gln14-Arg683) was expressed in mammalian cells with a His tag and Avi at the C-terminus. Accession: QHD43416.1
形式 The biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
纯度 > 95% by PAGE under reduced condition, and SEC-HPLC.
内毒素 Less than 1 EU per ug by the LAL method.
运输 The biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
注意事项 His-tagged and Fc-fused SARS-CoV-2 Spike protein S1 subunits are available. Please ask for a quote if you need spike protein S1 subunit mutants or a spike protein S1 subunit with different modification or components.
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描述

BP003074: Biotinylated Recombinant SARS Spike S1-Fc-Avi Protein

Background

Source: recombinant SARS-CoV-2 Spike S1 protein (S1 subunit, Gln14-Arg683) was expressed in mammalian cells with a His tag and Avi at the C-terminus.
Accession: QHD43416.1
Predicted molecular mass: 77.9 kDa. Due to glycosylation, the biotinylated recombinant SARS-CoV-2 Spike S1 protein migrates to 110-120 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 1 EU per ug by the LAL method.
Activity: Immobilized biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein at 1 ug/ml (100 ul/well). Dose response curve for the recombinant human ACE2-Fc fusion protein with the EC50 of 81 ng/ml determined by ELISA.
Formulation: The biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.

Shipping: The biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

The spike protein (S protein) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on the S protein mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services

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