Mouse FLT3 Ligand Protein C-His Tag | Syd Labs BP003497
Syd Labs 货号:BP003497 Mouse FLT3 Ligand Protein with the C-His Tag (小鼠 FLT3 配体蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Gly27-Gln189.
产品参数
| 货号 | BP003497 |
|---|---|
| 产品名称 | Mouse FLT3 Ligand Protein C-His Tag | Syd Labs BP003497 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Mouse |
| 来源 | The recombinant Mouse FLT3 Ligand Protein, (amino acids Gly27-Gln189) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Gly27-Gln189 |
| 分子量 | The recombinant Mouse FLT3 Ligand protein with the His tag at the C-terminus has a predicted MW of 19.61 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Mouse FLT3 Ligand protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human FLT3 protein with the C-hFc tag with the EC50 of 39.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Mouse FLT3 Ligand Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Mouse FLT3 Ligand Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Mouse FLT3 Ligand Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品,不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题,欢迎随时联系Syd Labs国内市场推广合作伙伴:武汉多找找科技有限公司,企业微信:duozhaozhao2024 联系电话:18162581039(龙经理) |
文献
BP003497: Mouse FLT3 Ligand Protein with the C-His Tag (小鼠 FLT3 配体蛋白 C-His 标签)
Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
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