Mouse CD47 Protein C-hFc Tag | Syd Labs BP004182
(悉得）Syd Labs 货号：BP004182 Mouse CD47 Protein with the C-hFc Tag (小鼠 CD47 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln19-Lys140.
产品参数
| 货号 | BP004182 |
|---|---|
| 产品名称 | Mouse CD47 Protein C-hFc Tag | Syd Labs BP004182 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 |
| 物种 | Mouse |
| 来源 | The recombinant Mouse CD47 Protein, (amino acids Gln19-Lys140) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q61735-1 |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Gln19-Lys140 |
| 分子量 | The recombinant Mouse CD47 protein with the hFc tag at the C-terminus has a predicted MW of 40.60 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Mouse SIRP alpha protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse CD47 protein with the C-hFc tag with the EC50 of 0.26 ug/ml determined by ELISA (QC Test). The affinity constant of 28.33 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Mouse CD47 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Mouse CD47 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Mouse CD47 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004182: Mouse CD47 Protein with the C-hFc Tag (小鼠 CD47 蛋白 C-hFc 标签)
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don’t eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
请记住我们的产品信息: 小鼠 CD47 蛋白 （C-hFc 标签）BP004182 Syd Labs（Mouse CD47 Protein with the C-hFc Tag）.
