Mouse ARTN Protein N-hFc Tag | Syd Labs BP004809
(悉得）Syd Labs 货号：BP004809 Mouse ARTN Protein with the N-hFc Tag (小鼠 ARTN 蛋白 N-hFc 标签) is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala112-Gly224.
产品参数
| 货号 | BP004809 |
|---|---|
| 产品名称 | Mouse ARTN Protein N-hFc Tag | Syd Labs BP004809 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN |
| 物种 | Mouse |
| 来源 | The recombinant Mouse ARTN Protein, (amino acids Ala112-Gly224) fused with a hFc tag at the N-terminus, was produced in HEK 293 cells. |
| 产品标签 | N-hFc |
| 生物素化 | no |
| 确定序列 | Ala112-Gly224 |
| 分子量 | The recombinant Mouse ARTN protein with the hFc tag at the N-terminus has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Mouse ARTN protein with the N-hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse GFRA3 protein with the N-His tag with the EC50 of 32.2 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Mouse ARTN Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Mouse ARTN Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Mouse ARTN Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004809: Mouse ARTN Protein with the N-hFc Tag (小鼠 ARTN 蛋白 N-hFc 标签)
Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.
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