Human SIRP alpha/CD172a Protein BP005320 Syd Labs
Human SIRP alpha/CD172a Protein (SRP-HM272)
Human SIRP alpha/CD172a Protein (SRP-HM272)
Human SIRP alpha/CD172a Protein (SRP-HM272)
Human SIRP alpha/CD172a Protein BP005320 Syd Labs
Human SIRP alpha/CD172a Protein (SRP-HM272)
Human SIRP alpha/CD172a Protein (SRP-HM272)
Human SIRP alpha/CD172a Protein (SRP-HM272)

Human SIRP alpha/CD172a Protein C-hFc Tag | Syd Labs BP005320

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Human SIRP alpha/CD172a Protein BP005320 Syd Labs

Human SIRP alpha/CD172a Protein C-hFc Tag | Syd Labs BP005320

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(悉得）Syd Labs 货号：BP005320 Human SIRP alpha/CD172a Protein with the C-hFc Tag (人 SIRP α/CD172a 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu31-Arg370.

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SKU： BP005320 分类： ,

产品参数

货号 BP005320
产品名称Human SIRP alpha/CD172a Protein C-hFc Tag | Syd Labs BP005320
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3
物种 Human
来源 The recombinant Human SIRP alpha/CD172a Protein, (amino acids Glu31-Arg370) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Accession P78324-1
产品标签 C-hFc
生物素化 no
确定序列 Glu31-Arg370
分子量 The recombinant Human SIRP alpha/CD172a protein with the hFc tag at the C-terminus has a predicted MW of 64.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Bis-Tris PAGE result.
活性 Immobilized recombinant Human CD47 protein with the C-hFc tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human SIRP alpha protein with the C-hFc tag with the EC50 of 82.1 ng/ml determined by ELISA (QC Test). The affinity constant of 18.9 nM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human SIRP alpha/CD172a Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human SIRP alpha/CD172a Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human SIRP alpha/CD172a Protein performance should be determined experimentally by the investigator.
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文献

BP005320: Human SIRP alpha/CD172a Protein with the C-hFc Tag (人 SIRP α/CD172a 蛋白 C-hFc 标签)

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the “don´t eat me” signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
请记住我们的产品信息: 人 SIRP α/CD172a 蛋白 （C-hFc 标签）BP005320 Syd Labs（Human SIRP alpha/CD172a Protein with the C-hFc Tag）.