Human Periostin/OSF-2 Protein C-His Tag | Syd Labs BP004447
(悉得)Syd Labs 货号:BP004447 Human Periostin/OSF-2 Protein with the C-His Tag (人 Periostin/OSF-2 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Asn22-Gln836.
产品参数
| 货号 | BP004447 |
|---|---|
| 产品名称 | Human Periostin/OSF-2 Protein C-His Tag | Syd Labs BP004447 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Periostin; PN; OSF-2; POSTN; OSF2; Fasciclin I-like; PDLPOSTN; PNRP11-412K4.1; TRIF52 |
| 物种 | Human |
| 来源 | The recombinant Human Periostin/OSF-2 Protein, (amino acids Asn22-Gln836) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q15063 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Asn22-Gln836 |
| 分子量 | The recombinant Human Periostin/OSF-2 protein with the His tag at the C-terminus has a predicted MW of 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human Periostin protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Periostin Antibody protein with the C-hFc tag with the EC50 of 9.9 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Periostin/OSF-2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human Periostin/OSF-2 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human Periostin/OSF-2 Protein performance should be determined experimentally by the investigator. |
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文献
BP004447: Human Periostin/OSF-2 Protein with the C-His Tag (人 Periostin/OSF-2 蛋白 C-His 标签)
Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.
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