Human PDGF R alpha/PDGFRA Protein C-His-Avi Tag | Syd Labs BP005608
(悉得)Syd Labs 货号:BP005608 Human PDGF R alpha/PDGFRA Protein with the C-His-Avi Tag (人 PDGF R α/PDGFRA 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Glu524.
产品参数
| 货号 | BP005608 |
|---|---|
| 产品名称 | Human PDGF R alpha/PDGFRA Protein C-His-Avi Tag | Syd Labs BP005608 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | PDGFRA; CD140A; PDGFR2; RHEPDGFRA; MGC74795; PDGFR-2 |
| 物种 | Human |
| 来源 | The recombinant Human PDGF R alpha/PDGFRA Protein, (amino acids Gln24-Glu524) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | P16234 |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Gln24-Glu524 |
| 分子量 | The recombinant Human PDGF R alpha/PDGFRA protein with the His-Avi tag at the C-terminus has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human PDGF R alpha protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-PDGF R alpha Antibody protein with the C-hFc tag with the EC50 of 4.6 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human PDGF R alpha/PDGFRA Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human PDGF R alpha/PDGFRA Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human PDGF R alpha/PDGFRA Protein performance should be determined experimentally by the investigator. |
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文献
BP005608: Human PDGF R alpha/PDGFRA Protein with the C-His-Avi Tag (人 PDGF R α/PDGFRA 蛋白 C-His-Avi 标签)
Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF.
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