Human NKG2A&CD94 Protein N-His-Avi Tag | Syd Labs BP005621

Human NKG2A&CD94 Protein BP005621 Syd Labs

Human NKG2A&CD94 Protein N-His-Avi Tag | Syd Labs BP005621

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Syd Labs 货号:BP005621 Human NKG2A&CD94 Protein with the N-His-Avi Tag (人 NKG2A&CD94 蛋白 N-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).

SKU: BP005621 分类: ,

产品参数

货号 BP005621
产品名称Human NKG2A&CD94 Protein N-His-Avi Tag | Syd Labs BP005621
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
物种 Human
来源 The recombinant Human NKG2A&CD94 Protein, (amino acids Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
产品标签 N-His-Avi
生物素化 no
确定序列 Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
分子量 The recombinant Human NKG2A&CD94 protein with the His-Avi tag at the N-terminus has a predicted MW of 17.9 kDa (CD94) and 18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human NKG2A&CD94 protein with the N-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-NKG2A Antibody protein with the N-hFc tag with the EC50 of 2.9 ng/ml determined by ELISA (QC Test).
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human NKG2A&CD94 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human NKG2A&CD94 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human NKG2A&CD94 Protein performance should be determined experimentally by the investigator.
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文献

BP005621: Human NKG2A&CD94 Protein with the N-His-Avi Tag (人 NKG2A&CD94 蛋白 N-His-Avi 标签)

 

The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

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