Human MSLN/Mesothelin Protein C-His-Avi Tag | Syd Labs BP005311
Syd Labs 货号：BP005311 Human MSLN/Mesothelin Protein with the C-His-Avi Tag (人 MSLN/间皮素蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu296-Gly580.
产品参数
| 货号 | BP005311 |
|---|---|
| 产品名称 | Human MSLN/Mesothelin Protein C-His-Avi Tag | Syd Labs BP005311 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR |
| 物种 | Human |
| 来源 | The recombinant Human MSLN/Mesothelin Protein, (amino acids Glu296-Gly580) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Glu296-Gly580 |
| 分子量 | The recombinant Human MSLN/Mesothelin protein with the His-Avi tag at the C-terminus has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human MSLN protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well). Dose response curve for the recombinant Anti-MSLN Antibody protein with the C-hFc tag with the EC50 of 12.4 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human MSLN/Mesothelin Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human MSLN/Mesothelin Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human MSLN/Mesothelin Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005311: Human MSLN/Mesothelin Protein with the C-His-Avi Tag (人 MSLN/间皮素蛋白 C-His-Avi 标签)
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
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