Human LILRB1/CD85j/ILT2 Domain1&2 Protein C-His Tag | Syd Labs BP005249
(悉得）Syd Labs 货号：BP005249 Human LILRB1/CD85j/ILT2 Domain1&2 Protein with the C-His Tag (人 LILRB1/CD85j/ILT2 结构域 1&2 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Gly221.
产品参数
| 货号 | BP005249 |
|---|---|
| 产品名称 | Human LILRB1/CD85j/ILT2 Domain1&2 Protein C-His Tag | Syd Labs BP005249 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4 |
| 物种 | Human |
| 来源 | The recombinant Human LILRB1/CD85j/ILT2 Domain1&2 Protein, (amino acids Gly24-Gly221) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Gly24-Gly221 |
| 分子量 | The recombinant Human LILRB1/CD85j/ILT2 Domain1&2 protein with the His tag at the C-terminus has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human LILRB1 Domain1&2 protein with the C-His tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-LILRB1 Antibody protein with the C-hFc tag with the EC50 of 16.9 ng/ml determined by ELISA (QC Test). The affinity constant of 1.9 uM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human LILRB1/CD85j/ILT2 Domain1&2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human LILRB1/CD85j/ILT2 Domain1&2 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human LILRB1/CD85j/ILT2 Domain1&2 Protein performance should be determined experimentally by the investigator. |
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文献
BP005249: Human LILRB1/CD85j/ILT2 Domain1&2 Protein with the C-His Tag (人 LILRB1/CD85j/ILT2 结构域 1&2 蛋白 C-His 标签)
LILRB1, also known as CD85j and IL-T2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens.
请记住我们的产品信息: 人 LILRB1/CD85j/ILT2 结构域 1&2 蛋白 （C-His 标签）BP005249 Syd Labs（Human LILRB1/CD85j/ILT2 Domain1&2 Protein with the C-His Tag）.