Human LIGHT/TNFSF14 Trimer Protein N-His-Flag Tag | Syd Labs BP005350
(悉得）Syd Labs 货号：BP005350 Human LIGHT/TNFSF14 Trimer Protein with the N-His-Flag Tag (人 LIGHT/TNFSF14 三聚体蛋白 N-His-Flag 标签) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ser89-Val240 Trimer.
产品参数
| 货号 | BP005350 |
|---|---|
| 产品名称 | Human LIGHT/TNFSF14 Trimer Protein N-His-Flag Tag | Syd Labs BP005350 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD258; TNFSF14; HVEM-L; LIGHT; LTg; TR2 |
| 物种 | Human |
| 来源 | The recombinant Human LIGHT/TNFSF14 Trimer Protein, (amino acids Ser89-Val240) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells. |
| 产品标签 | N-His-Flag |
| 生物素化 | no |
| 确定序列 | Ser89-Val240 |
| 分子量 | The recombinant Human LIGHT/TNFSF14 Trimer protein with the His-Flag tag at the N-terminus has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-140 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human LIGHT Trimer protein with the N-His-Flag tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human HVEM protein with the N-hFc tag with the EC50 of 86.3 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human LIGHT/TNFSF14 Trimer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human LIGHT/TNFSF14 Trimer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human LIGHT/TNFSF14 Trimer Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005350: Human LIGHT/TNFSF14 Trimer Protein with the N-His-Flag Tag (人 LIGHT/TNFSF14 三聚体蛋白 N-His-Flag 标签)
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
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