Human IL-18 Protein No Tag | Syd Labs BP005730
(悉得）Syd Labs 货号：BP005730 Human IL-18 Protein with No Tag (人 IL-18 蛋白 No Tag) is expressed from E.coli without tag.It contains Tyr37-Asp193.
产品参数
| 货号 | BP005730 |
|---|---|
| 产品名称 | Human IL-18 Protein No Tag | Syd Labs BP005730 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1 |
| 物种 | Human |
| 来源 | The recombinant Human IL-18 Protein, (amino acids Tyr37-Asp193) fused with No Tag, was produced in E.coli cells. |
| 产品标签 | No Tag |
| 生物素化 | no |
| 确定序列 | Tyr37-Asp193 |
| 分子量 | The recombinant Human IL-18 Protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human IL-18 at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human IL-18BP, hFc tag with the EC50 of 16.9 ng/ml determined by ELISA (QC Test). The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human IL-18 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human IL-18 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human IL-18 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005730: Human IL-18 Protein with No Tag (人 IL-18 蛋白 No Tag)
Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
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