Human IL-15RA&IL-15 Protein C-His Tag | Syd Labs BP004351
(悉得）Syd Labs 货号：BP004351 Human IL-15RA&IL-15 Protein with the C-His Tag (人 IL-15RA&IL-15 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).
产品参数
| 货号 | BP004351 |
|---|---|
| 产品名称 | Human IL-15RA&IL-15 Protein C-His Tag | Syd Labs BP004351 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | IL-15 R alpha；CD215；IL15RA；IL-15RA；IL-15R-alpha1；Interleukin-15；IL-15；IL15 |
| 物种 | Human |
| 来源 | The recombinant Human IL-15RA&IL-15 Protein, (amino acids Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q13261-1(IL-15RA)&P40933-1(IL-15) |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15) |
| 分子量 | The recombinant Human IL-15RA&IL-15 protein with the His tag at the C-terminus has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human IL-15RA&IL-15 protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IL-2 R beta&IL-2 R gamma protein with the C-hFc tag with the EC50 of 38.8 ng/ml determined by ELISA (QC Test). The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human IL-15RA&IL-15 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1EU per ug by the LAL method. |
| 运输 | The recombinant Human IL-15RA&IL-15 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human IL-15RA&IL-15 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004351: Human IL-15RA&IL-15 Protein with the C-His Tag (人 IL-15RA&IL-15 蛋白 C-His 标签)
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
请记住我们的产品信息: 人 IL-15RA&IL-15 蛋白 （C-His 标签）BP004351 Syd Labs（Human IL-15RA&IL-15 Protein with the C-His Tag）.
