Human IL-12B/p40/NKSF2 Protein C-His Tag | Syd Labs BP005179
(悉得）Syd Labs 货号：BP005179 Human IL-12B/p40/NKSF2 Protein with the C-His Tag (人 IL-12B/p40/NKSF2 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Ser328.
产品参数
| 货号 | BP005179 |
|---|---|
| 产品名称 | Human IL-12B/p40/NKSF2 Protein C-His Tag | Syd Labs BP005179 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | IL-12B; CLMF p40; IL-12 subunit p40; NKSF2; CLMF; CLMF2; NKS |
| 物种 | Human |
| 来源 | The recombinant Human IL-12B/p40/NKSF2 Protein, (amino acids Ile23-Ser328) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | P29460 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Ile23-Ser328 |
| 分子量 | The recombinant Human IL-12B/p40/NKSF2 protein with the His tag at the C-terminus has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human IL-12B protein with the C-His tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-IL-12 Antibody protein with the C-hFc tag with the EC50 of 18.5 ng/ml determined by ELISA. The affinity constant of 98.1 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human IL-12B/p40/NKSF2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human IL-12B/p40/NKSF2 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human IL-12B/p40/NKSF2 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005179: Human IL-12B/p40/NKSF2 Protein with the C-His Tag (人 IL-12B/p40/NKSF2 蛋白 C-His 标签)
Interleukin (IL)‑12B, which encodes the p40 subunit common to IL‑12 and IL‑23, as one of the genes for which expression in fibroblast‑like synoviocytes from patients with rheumatoid arthritis (RA‑FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.
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