Human HVEM/TNFRSF14 Protein C-hFc Tag | Syd Labs BP005357
(悉得）Syd Labs 货号：BP005357 Human HVEM/TNFRSF14 Protein with the C-hFc Tag (人 HVEM/TNFRSF14 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu39-Val202.
产品参数
| 货号 | BP005357 |
|---|---|
| 产品名称 | Human HVEM/TNFRSF14 Protein C-hFc Tag | Syd Labs BP005357 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD270; CD40-like protein; HVEM; LIGHTR; ATAR; TNFRSF14; TR2 |
| 物种 | Human |
| 来源 | The recombinant Human HVEM/TNFRSF14 Protein, (amino acids Leu39-Val202) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q92956-1 |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Leu39-Val202 |
| 分子量 | The recombinant Human HVEM/TNFRSF14 protein with the hFc tag at the C-terminus has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human LIGHT protein with the C-hFc tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human HVEM protein with the C-hFc tag with the EC50 of 15.3 ng/ml determined by ELISA. The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human HVEM/TNFRSF14 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human HVEM/TNFRSF14 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human HVEM/TNFRSF14 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005357: Human HVEM/TNFRSF14 Protein with the C-hFc Tag (人 HVEM/TNFRSF14 蛋白 C-hFc 标签)
Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
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