Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein C-His-Avi Tag | Syd Labs BP005412
(悉得）Syd Labs 货号：BP005412 Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein with the C-His-Avi Tag (人 HLA-A*02：01&B2M&GP100 （YLEPGPVTA） 单体蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide.
产品参数
| 货号 | BP005412 |
|---|---|
| 产品名称 | Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein C-His-Avi Tag | Syd Labs BP005412 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100 |
| 物种 | Human |
| 来源 | The recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein, (amino acids Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide |
| 分子量 | The recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer protein with the His-Avi tag at the C-terminus has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer protein with the C-His-Avi tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody protein with the C-hFc tag with the EC50 of 9.1 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005412: Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein with the C-His-Avi Tag (人 HLA-A*02：01&B2M&GP100 （YLEPGPVTA） 单体蛋白 C-His-Avi 标签)
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
请记住我们的产品信息: 人 HLA-A*02：01&B2M&GP100 （YLEPGPVTA） 单体蛋白 （C-His-Avi 标签）BP005412 Syd Labs（Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein with the C-His-Avi Tag）.
