Human GPC3/Glypican 3 Protein C-His Tag | Syd Labs BP005337
(悉得)Syd Labs 货号:BP005337 Human GPC3/Glypican 3 Protein with the C-His Tag (人 GPC3/Glypican 3 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-His559.
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货号 | BP005337 |
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产品名称 | Human GPC3/Glypican 3 Protein C-His Tag | Syd Labs BP005337 |
供货商名称 | Syd Labs, Inc. |
品牌名 | 悉得(Syd Labs) |
别称 | GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1 |
物种 | Human |
来源 | The recombinant Human GPC3/Glypican 3 Protein, (amino acids Gln25-His559) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
Accession | P51654-1 |
产品标签 | C-His |
生物素化 | no |
确定序列 | Gln25-His559 |
分子量 | The recombinant Human GPC3/Glypican 3 protein with the His tag at the C-terminus has a predicted MW of 61.6 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 42 kDa, 70-115 kDa based on Tris-Bis PAGE result. |
活性 | Immobilized recombinant Human GPC3 protein with the C-His tag at 0.5 ug/ml (100 ul/Well). Dose response curve for the recombinant Anti-GPC3 Antibody protein with the C-hFc tag with the EC50 of 4.7 ng/ml determined by ELISA. |
Form | Lyophilized |
形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
制剂 | It is recommended to reconstitute the lyophilized recombinant Human GPC3/Glypican 3 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
运输 | The recombinant Human GPC3/Glypican 3 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
注意事项 | Conditions of optimal recombinant Human GPC3/Glypican 3 Protein performance should be determined experimentally by the investigator. |
线下下单 | 武汉多找找科技有限公司 电话: 18162581039 微信: duozhaozhao2024 Email: message@sydlabs.com或联系我们 |
描述
BP005337: Human GPC3/Glypican 3 Protein with the C-His Tag (人 GPC3/Glypican 3 蛋白 C-His 标签)
Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
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