Human GM-CSF R alpha Protein C-His Tag | Syd Labs BP004976
Syd Labs 货号：BP004976 Human GM-CSF R alpha Protein with the C-His Tag (人 GM-CSF R α 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Glu23-Gly320.
产品参数
| 货号 | BP004976 |
|---|---|
| 产品名称 | Human GM-CSF R alpha Protein C-His Tag | Syd Labs BP004976 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4 |
| 物种 | Human |
| 来源 | The recombinant Human GM-CSF R alpha Protein, (amino acids Glu23-Gly320) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Glu23-Gly320 |
| 分子量 | The recombinant Human GM-CSF R alpha protein with the His tag at the C-terminus has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human GM-CSF protein with the C-His tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human GM-CSF R alpha protein with the C-His tag with the EC50 of 1.0 ug/ml determined by ELISA. The affinity constant of 0.79 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human GM-CSF R alpha Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human GM-CSF R alpha Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
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文献
BP004976: Human GM-CSF R alpha Protein with the C-His Tag (人 GM-CSF R α 蛋白 C-His 标签)
In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
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