Human FOLR1 Protein C-hFc Tag | Syd Labs BP005026
(悉得)Syd Labs 货号:BP005026 Human FOLR1 Protein with the C-hFc Tag (人 FOLR1 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Arg25-Met233.
产品参数
| 货号 | BP005026 |
|---|---|
| 产品名称 | Human FOLR1 Protein C-hFc Tag | Syd Labs BP005026 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | FOLR; FOLR1; FBP; Folbp1; KB cells FBP; MOv18 |
| 物种 | Human |
| 来源 | The recombinant Human FOLR1 Protein, (amino acids Arg25-Met233) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Arg25-Met233 |
| 分子量 | The recombinant Human FOLR1 protein with the hFc tag at the C-terminus has a predicted MW of 51.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human FOLR1 protein with the C-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-FOLR1 Antibody protein with the C-hFc tag with the EC50 of 25.4 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human FOLR1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human FOLR1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human FOLR1 Protein performance should be determined experimentally by the investigator. |
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文献
BP005026: Human FOLR1 Protein with the C-hFc Tag (人 FOLR1 蛋白 C-hFc 标签)
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 – 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH.
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