Human Fc epsilon RI alpha/FCER1a Protein C-hFc Tag | Syd Labs BP004551
(悉得）Syd Labs 货号：BP004551 Human Fc epsilon RI alpha/FCER1a Protein with the C-hFc Tag (人 Fc ε RI alpha/FCER1a 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val26-Gln205.
产品参数
| 货号 | BP004551 |
|---|---|
| 产品名称 | Human Fc epsilon RI alpha/FCER1a Protein C-hFc Tag | Syd Labs BP004551 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Fc-epsilon RI-alpha; FcERI; FCER1A; FCE1A |
| 物种 | Human |
| 来源 | The recombinant Human Fc epsilon RI alpha/FCER1a Protein, (amino acids Val26-Gln205) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Val26-Gln205 |
| 分子量 | The recombinant Human Fc epsilon RI alpha/FCER1a protein with the hFc tag at the C-terminus has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human Fc epsilon RI alpha protein with the C-hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Anti-Fc epsilon RI alpha Antibody protein with the C-hFc tag with the EC50 14.5 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Fc epsilon RI alpha/FCER1a Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human Fc epsilon RI alpha/FCER1a Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human Fc epsilon RI alpha/FCER1a Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004551: Human Fc epsilon RI alpha/FCER1a Protein with the C-hFc Tag (人 Fc ε RI alpha/FCER1a 蛋白 C-hFc 标签)
Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
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