Human ENPP-1 Protein N-His Tag | Syd Labs BP004291
(悉得）Syd Labs 货号：BP004291 Human ENPP-1 Protein with the N-His Tag (人 ENPP-1 蛋白 N-His 标签) is expressed from HEK293 with His tag at the N-Terminus.It contains Lys98-Asp925.
产品参数
| 货号 | BP004291 |
|---|---|
| 产品名称 | Human ENPP-1 Protein N-His Tag | Syd Labs BP004291 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2 |
| 物种 | Human |
| 来源 | The recombinant Human ENPP-1 Protein, (amino acids Lys98-Asp925) fused with a His tag at the N-terminus, was produced in HEK 293 cells. |
| 产品标签 | N-His |
| 生物素化 | no |
| 确定序列 | Lys98-Asp925 |
| 分子量 | The recombinant Human ENPP-1 protein with the His tag at the N-terminus has a predicted MW of 96.5 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human ENPP-1 protein with the N-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-ENPP-1 Antibody protein with the N-hFc tag with the EC50 of 17.7 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human ENPP-1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human ENPP-1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. |
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文献
BP004291: Human ENPP-1 Protein with the N-His Tag (人 ENPP-1 蛋白 N-His 标签)
Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.
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