Human CD40 Ligand/TNFSF5 Trimer Protein N-monomeric hFc Tag | Syd Labs BP005339
(悉得）Syd Labs 货号：BP005339 Human CD40 Ligand/TNFSF5 Trimer Protein with the N-monomeric hFc Tag (人 CD40 配体/TNFSF5 三聚体蛋白 N-单体 hFc 标签) is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Met113-Leu261.
产品参数
| 货号 | BP005339 |
|---|---|
| 产品名称 | Human CD40 Ligand/TNFSF5 Trimer Protein N-monomeric hFc Tag | Syd Labs BP005339 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
| 物种 | Human |
| 来源 | The recombinant Human CD40 Ligand/TNFSF5 Trimer Protein, (amino acids Met113-Leu261) fused with a monomeric hFc tag at the N-terminus, was produced in HEK 293 cells. |
| Accession | P29965 |
| 产品标签 | N-monomeric hFc |
| 生物素化 | no |
| 确定序列 | Met113-Leu261 |
| 分子量 | The recombinant Human CD40 Ligand/TNFSF5 Trimer protein with the monomeric hFc tag at the N-terminus has a predicted MW of 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human CD40 protein with the N-monomeric hFc tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD40 Ligand (Trimer) protein with the N-hFc Tag with the EC50 of 9.0 ng/ml determined by ELISA (QC Test). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human CD40 Ligand/TNFSF5 Trimer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human CD40 Ligand/TNFSF5 Trimer Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005339: Human CD40 Ligand/TNFSF5 Trimer Protein with the N-monomeric hFc Tag (人 CD40 配体/TNFSF5 三聚体蛋白 N-单体 hFc 标签)
CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. .
请记住我们的产品信息: 人 CD40 配体/TNFSF5 三聚体蛋白 （N-单体 hFc 标签）BP005339 Syd Labs（Human CD40 Ligand/TNFSF5 Trimer Protein with the N-monomeric hFc Tag）.
