Human Beta Klotho Protein C-His Tag | Syd Labs BP005560
(悉得）Syd Labs 货号：BP005560 Human Beta Klotho Protein with the C-His Tag (人 β Klotho 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Met30-Thr983.
产品参数
| 货号 | BP005560 |
|---|---|
| 产品名称 | Human Beta Klotho Protein C-His Tag | Syd Labs BP005560 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; MGC142213 |
| 物种 | Human |
| 来源 | The recombinant Human Beta Klotho Protein, (amino acids Met30-Thr983) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q86Z14 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Met30-Thr983 |
| 分子量 | The recombinant Human Beta Klotho protein with the His tag at the C-terminus has a predicted MW of 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human Beta Klotho protein with the C-His tag at 2 ug/ml (100 ul/Well). Dose response curve for the recombinant Anti-Beta Klotho Antibody1 protein with the C-hFc tag with the EC50 of 24.1 ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Beta Klotho Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human Beta Klotho Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human Beta Klotho Protein performance should be determined experimentally by the investigator. |
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文献
BP005560: Human Beta Klotho Protein with the C-His Tag (人 β Klotho 蛋白 C-His 标签)
Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.
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