Human Alkaline phosphatase (Intestinal type) /ALPI Protein N-His-Flag Tag | Syd Labs BP005211
(悉得）Syd Labs 货号：BP005211 Human Alkaline phosphatase (Intestinal type) /ALPI Protein with the N-His-Flag Tag (人碱性磷酸酶（肠道型）/ALPI 蛋白 N-His-Flag 标签) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Val20-Asp503.
产品参数
| 货号 | APE-HM101 |
|---|---|
| 产品名称 | Human Alkaline phosphatase (Intestinal type) /ALPI Protein N-His-Flag Tag | Syd Labs BP005211 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | IAP; Akp2; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP |
| 物种 | Human |
| 来源 | The recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein, (amino acids Val20-Asp503) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells. |
| 产品标签 | N-His-Flag |
| 生物素化 | no |
| 确定序列 | Val20-Asp503 |
| 分子量 | The recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein with the His-Flag tag at the N-terminus has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result. |
| 活性 | The affinity constant of 1.58 uM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal Human Alkaline phosphatase (Intestinal type) /ALPI Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005211: Human Alkaline phosphatase (Intestinal type) /ALPI Protein with the N-His-Flag Tag (人碱性磷酸酶（肠道型）/ALPI 蛋白 N-His-Flag 标签)
Alkaline phosphatase can be considered “our favorite enzyme” for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
请记住我们的产品信息: 人碱性磷酸酶（肠道型）/ALPI 蛋白 （N-His-Flag 标签）BP005211 Syd Labs（Human Alkaline phosphatase (Intestinal type) /ALPI Protein with the N-His-Flag Tag）.