Cynomolgus HGF R/c-MET Protein C-His Tag | Syd Labs BP003907
(悉得）Syd Labs 货号：BP003907 Cynomolgus HGF R/c-MET Protein with the C-His Tag (食蟹猴 HGF R/c-MET 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Asn930.
产品参数
| 货号 | BP003907 |
|---|---|
| 产品名称 | Cynomolgus HGF R/c-MET Protein C-His Tag | Syd Labs BP003907 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Cynomolgus |
| 来源 | The recombinant Cynomolgus HGF R/c-MET Protein, (amino acids Glu25-Asn930) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Glu25-Asn930 |
| 分子量 | The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The recombinant Cynomolgus HGF R/c-MET protein with the His tag at the C-terminus has a predicted MW of 32.5 kDa (α chain) and 69.9 kDa (β chain). Due to glycosylation, the protein migrates to 45-52 kDa (α chain) and 75-82 kDa (β chain) based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Cynomolgus HGF R protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-HGF R Antibody protein with the C-hFc tag with the EC50 of 38.4 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Cynomolgus HGF R/c-MET Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Cynomolgus HGF R/c-MET Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Cynomolgus HGF R/c-MET Protein performance should be determined experimentally by the investigator. |
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文献
BP003907: Cynomolgus HGF R/c-MET Protein with the C-His Tag (食蟹猴 HGF R/c-MET 蛋白 C-His 标签)
c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.
请记住我们的产品信息: 食蟹猴 HGF R/c-MET 蛋白 （C-His 标签）BP003907 Syd Labs（Cynomolgus HGF R/c-MET Protein with the C-His Tag）.
