Cynomolgus GARP&Latent TGF beta 1 Complex Protein C-His-Avi Tag | Syd Labs BP005169
(悉得）Syd Labs 货号：BP005169 Cynomolgus GARP&Latent TGF beta 1 Complex Protein with the C-His-Avi Tag (食蟹猴 GARP&Latent TGF beta 1 复合蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala18-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1).
产品参数
| 货号 | BP005169 |
|---|---|
| 产品名称 | Cynomolgus GARP&Latent TGF beta 1 Complex Protein C-His-Avi Tag | Syd Labs BP005169 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1 |
| 物种 | Cynomolgus |
| 来源 | The recombinant Cynomolgus GARP&Latent TGF beta 1 Complex Protein, (amino acids Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1) |
| 分子量 | The recombinant Cynomolgus GARP&Latent TGF beta 1 Complex protein with the His-Avi tag at the C-terminus has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta 1) based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Cynomolgus GARP&Latent TGF beta 1 Complex protein with the C-His-Avi tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-GARP&Latent TGF Beta 1 Antibody protein with the C-hFc tag with the EC50 of 10.1 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Cynomolgus GARP&Latent TGF beta 1 Complex Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Cynomolgus GARP&Latent TGF beta 1 Complex Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Cynomolgus GARP&Latent TGF beta 1 Complex Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005169: Cynomolgus GARP&Latent TGF beta 1 Complex Protein with the C-His-Avi Tag (食蟹猴 GARP&Latent TGF beta 1 复合蛋白 C-His-Avi 标签)
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
请记住我们的产品信息: 食蟹猴 GARP&Latent TGF beta 1 复合蛋白 （C-His-Avi 标签）BP005169 Syd Labs（Cynomolgus GARP&Latent TGF beta 1 Complex Protein with the C-His-Avi Tag）.
