Biotinylated Human IL-12 Protein C-His-Avi Tag | Syd Labs BP005012

Biotinylated Human IL-12 Protein BP005012 Syd Labs

Biotinylated Human IL-12 Protein C-His-Avi Tag | Syd Labs BP005012

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Syd Labs 货号:BP005012 Biotinylated Human IL-12 Protein with the C-His-Avi Tag (生物素化人 IL-12 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A).

SKU: BP005012 分类: ,

产品参数

货号 BP005012
产品名称Biotinylated Human IL-12 Protein C-His-Avi Tag | Syd Labs BP005012
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12
物种 Human
来源 The recombinant Biotinylated Human IL-12 Protein, (amino acids Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-His-Avi
生物素化 yes
确定序列 Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A)
分子量 The recombinant Biotinylated Human IL-12 protein with the His-Avi tag at the C-terminus has a predicted MW of 34.7 kDa (IL-12B) and 25.5 kDa (IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Anti-IL-12 Antibody protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-12 protein with the C-His tag with the EC50 of 10.8 ng/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Biotinylated Human IL-12 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Biotinylated Human IL-12 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Biotinylated Human IL-12 Protein performance should be determined experimentally by the investigator.
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文献

BP005012: Biotinylated Human IL-12 Protein with the C-His-Avi Tag (生物素化人 IL-12 蛋白 C-His-Avi 标签)

 

Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

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